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Product Name:
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A/bovine/Ohio/B24OSU-439/2024 (H5N1), Tissue Culture Adapted
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Ownership statement:
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This reagent is the tangible property of the U.S. Government.
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Manufacturer:
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BEI Resources
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Taxonomy:
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Virus Classification: Orthomyxoviridae, Alphainfluenzavirus
Species: Influenza A virus
Strain/Isolate: A/bovine/Ohio/B24OSU-439/2024 (H5N1)
Clade: 2.3.4.4b1,2
Genotype: B3.131
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Additional Information:
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The detection of H5N1 influenza in dairy cows was first reported in March 2024. To date, four human cases of H5N1 have been recorded in workers exposed to infected dairy cows, however, human-to-human transmission has not been reported.3 Presently, there is no evidence that the influenza H5N1 A/bovine virus has acquired human virus receptor binding capability.4,5,6
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Material Provided:
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Each vial contains approximately 0.5 mL of cell lysate and supernatant from Madin-Darby canine kidney (MDCK) cells infected with influenza A virus, A/bovine/Ohio/B24OSU-439/2024 (H5N1).
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-59872 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Host: Madin-Darby canine kidney (MDCK) cells (ATCC® CCL-34)
Growth Medium: Eagle’s Minimum Essential Medium supplemented with 0.125% bovine serum albumin (BSA) and 1 µg/mL L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin, or equivalent
Infection: Cells should be 80% to 100% confluent
Incubation: 2 to 4 days at 37°C and 5% CO2
Cytopathic Effect: Cell rounding and sloughing
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Webby, R.J., Personal Communication.
2. GISAID
3. Centers for Disease Control and Prevention, Centers for Disease Control and Prevention, www.cdc.gov/bird-flu/situation-summary/index.html. Accessed 3 September 2024.
4. Chopra, P., et al. “Receptor Binding Specificity of a Bovine A(H5N1) Influenza Virus.” bioRxiv (2024): Jul 31:2024.07.30.605893. PubMed: 39131339.
5. Uyeki, T. M., et al “Highly Pathogenic Avian Influenza A (H5N1) Virus Infection in a Dairy Farm Worker.” N. Engl. J. Med. 390 (2024): 2028-2029. PubMed: 38700506.
6. Garg, S., et al. “Outbreak of Highly Pathogenic Avian Influenza A (H5N1) Viruses in U.S. Dairy Cattle and Detection of Two Human Cases — United States, 2024.” MMWR Morb. Mortal. Wkly. Rep. 273 (2024): 501-505. PubMed: 38814843.
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Citation:
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Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Influenza A Virus, A/bovine/Ohio/B24OSU-439/2024 (H5N1), Tissue Culture Adapted, NR-59872.”
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Biosafety Level:
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3
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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