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Product Name:
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240023
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Ownership statement:
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This reagent is the tangible property of the U.S. Government.
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Manufacturer:
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BEI Resources
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Taxonomy:
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Virus Classification: Peribunyaviridae, Orthobunyavirus oropoucheense Species: Oropouche Virus Strain: Isolate: 240023
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Additional Information:
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OROV is an arbovirus with a tripartite RNA genome, which consists of three single-stranded RNA segments of negative polarity: L (encodes RNA-dependent RNA polymerase), M (encodes envelope glycoproteins Gn and Gc) and S (encodes nucleoprotein N). OROV was first isolated in 1955 from the blood of a forestry worker in Melajo Reserve, Trinidad and Tobago, and is named for the nearby Oropouche River.3,4 OROV is a zoonotic disease causing periodic outbreaks in Central America, the Caribbean and throughout South America outside of its endemic Amazon region.2,3 In 2024, an increase in OROV cases originating in travelers from Brazil and Cuba was documented in the United States, as well as in Europe.3 Transmission to humans occurs primarily through Culicoides paraensis (biting midge) and secondary vectors Culex quinquefasciatus or Aedes aegypti.2,3
Symptoms of OROV fever, also referred to as sloth fever, originating from its isolation in Brazil in 1960 from a pale-throated sloth, are similar to those of dengue and chikungunya, which often leads to misdiagnosis of the source of the infection.2,5 A frequent disease pattern in OROV fever is a recurrence of symptoms within days to weeks after resolution of the initial illness.3 A few cases that have occurred in Brazil since 2023 are characterized by more severe disease and fetal sequelae.2 |
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Material Provided:
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Each vial contains approximately 1 mL of cell lysate and supernatant from Homo sapiens lung carcinoma epithelial cells infected with OROV, 240023.Note: If homogeneity is required for your intended use, please purify prior to initiating work. |
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Packing/Storage:
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NR-59930 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Host: Homo sapiens lung carcinoma epithelial cells (A549; ATCC® CCL-185™) Growth Medium: F-12K Medium (Kaighn’s Modification of Ham’s Medium) supplemented with 2% fetal bovine serum, or equivalent Infection: Cells should be 60% to 70% confluent Incubation: 6 to 10 days at 37°C and 5% CO2 Cytopathic Effect: Cell rounding and sloughing
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Russell, B.J., Personal Communication. 2. Morrison, A., et al. “Oropouche Virus Disease among U.S. Travelers – United States, 2024.” MMWR Morb. Mortal. Wkly. Rep. 73 (2024): 769-773. PubMed: 39236058. 3. Branda, F., M. Ciccozzi and F. Scarpa. “Oropouche Virus Presenting in Italy after Travel to Cuba.” New Microbes New Infect. 60-61 (2024): 101450. PubMed: 39100738. 4. Anderson, C. R., et al. “Oropouche Virus: A New Human Disease Agent from Trinidad, West Indies.” Am. J. Trop. Med. Hyg. 10 (1961): 574-578. PubMed: 13683183. 5. Nunes, M. R. T., et al. “Oropouche Virus Isolation, Southeast Brazil.” Emerg. Infect. Dis. 11 (2005): 1610-1613. PubMed: 16318707.
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Citation:
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Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Oropouche Virus, 240023, NR-59930."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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