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NR-623  Vaccinia virus, MVA/S, Recombinant Expressing SARS CoV Spike (S) Protein
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: MVA/S, Recombinant Expressing SARS CoV Spike (S) Protein
Organism: Vaccinia virus
Biosafety Level: 2
Availability Status: In Stock
Store at: -60°C or colder
Contributor: B Moss
Scientists at for-profit institutions must contact the National Institute of Allergy and Infectious Diseases (NIAID), Email: before the reagent can be released. Please specify the name, BEI catalog number and a brief description of the intended use of the reagent in your email to NIAID.

Quantity limit per order for this item is 1. This item can be ordered twice a year.

A cDNA clone containing the entire open reading frame encoding the S protein of the Urbani strain of SARS CoV was modified by introducing silent mutations that eliminated two poxvirus transcription termination motifs (TTTTTNT). The S gene was amplified and inserted in the pLW44 transfer vector, bringing it under the control of the vaccinia virus modified H5 early/late promoter. Recombinant MVA was made by transfecting the transfer plasmid into primary chick embryo fibroblast cells infected with MVA.

Each vial contains approximately 1 mL of cell lysate and supernatant from chicken embryo cells (SL-29, ATCC ® CRL-1590™) infected with vaccinia virus, MVA/S.

Additional information and tools are available at ViPR (Virus Pathogen Resource).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vaccinia Virus, MVA/S, Recombinant Expressing SARS CoV Spike (S) Protein, NR-623."
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

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