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Product Name:
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H241 (Tissue Culture Adapted)
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Manufacturer:
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BEI Resources
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Taxonomy:
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Virus Classification: Flaviviridae, Flavivirus
Species: Dengue Virus Type 4
Strain/Isolate: H241 (tissue culture adapted)
Derived from ATCC® VR-1490™
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Additional Information:
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Dengue virus causes the most common vector-borne viral disease of humans, with over 50 million cases in tropical and subtropical regions each year.3 The disease is now endemic in over 110 countries in the world, with Southeast Asia and the Western Pacific being the most seriously affected. Dengue disease is caused by one of four closely related, but antigenically distinct, serotypes (designated DEN-1 to -4).3 Infections produce a spectrum of clinical illness ranging from a nonspecific viral syndrome to severe and fatal hemorrhagic disease.4,5 Humans are the major host of dengue virus, with Aedes aegypti mosquitoes the principal vectors.
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Material Provided:
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Each vial contains approximately 1 mL of cell lysate and supernatant from Macaca mulatta kidney epithelial cells (LLC-MK2 derivative; ATCC® CCL-7.1™) infected with DEN-4, H241.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-86 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Host: LLC-MK2 derivative cells; ATCC® CCL-7.1™
Growth Medium: Eagle’s Minimum Essential Medium containing Earle’s Balanced Salt Solution, non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate and 1500 mg/L sodium bicarbonate supplemented with 2% fetal bovine serum, or equivalent
Infection: Cells should be 70% to 100% confluent
Incubation: 7 to 9 days at 37°C and 5% CO2
Cytopathic Effect: Cell rounding and degeneration
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Hammon, W. M., et al. “Viruses Associated with Epidemic Hemorrhagic Fevers of the Philippines and Thailand.” Science 131 (1960): 1102-1103. PubMed: 14399343.
2. Yip, A., et al. Novartis Institute for Tropical Diseases, Singapore, Direct Submission (2005).
3. Holmes, E. C. and S. S. Twiddy. “The Origin, Emergence and Evolutionary Genetics of Dengue Virus.” Infect. Genet. Evol. 3 (2003): 19-28. PubMed: 12797969.
4. Malavige, G. N., et al. “Dengue Viral Infections.” Postgrad. Med. J. 80 (2004): 588-601. PubMed: 15466994.
5. Kao, C.-L., et al. “Laboratory Diagnosis of Dengue Virus Infection: Current and Future Perspectives in Clinical Diagnosis and Public Health.” J. Microbiol. Immunol. Infect. 38 (2005): 5-16. PubMed: 15692621.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Dengue Virus Type 4, H241 (Tissue Culture Adapted), NR-86."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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