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NR-11  Escherichia coli, EDL933 (Serotype O157:H7)
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at no cost to registered researchers.
Organism: Escherichia coli
Designations: EDL933 (Serotype O157:H7)
Biosafety Level: 2
Availability Status: In Stock
Store at: -60°C or colder
Contributor: G Plunkett
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Escherichia coli (E. coli), strain EDL933 was isolated from raw hamburger meat implicated in a hemorrhagic colitis outbreak in the United States in 1982.

E. coli, strain EDL933 was originally deposited to the ATCC ® by the CDC in 1988 as ATCC ® 43895­. ATCC ® 43895 was acquired by the University of Wisconsin for use in a genome sequencing project and re-deposited to the ATCC ® as ATCC ® 700927.

The presence of the plasmid pO157 and chromosomal virulence markers stx1, stx2 and eaeA have been confirmed by PCR amplification of extracted DNA.

Each vial contains approximately 0.5 mL of bacterial culture in 0.5X Tryptic Soy Broth supplemented with 10% glycerol.

Note: If homogeneity is required for your intended use, please purify prior to initiating work.
Citations: Acknowledgment for publications should read "The following reagent was obtained through the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH: Escherichia coli, Strain EDL933, NR-11."
Publications Citing
this Reagent:
Wang, F. "Development and Application of Serogroup-Independent and Serogroup-Specific Loop-Mediated Isothermal Amplification Assays for Detecting Shiga Toxin-Producing Escherichia coli." Diss. China Agricultural University, 2011.
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Derived from:   ATCC 700927
Genomic DNA from Escherichia coli, Strain EDL933
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