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NR-12  Escherichia coli, RIMD 0509952 (Serotype O157:H7)
(Bacteria)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Escherichia coli
Designations: RIMD 0509952 (Serotype O157:H7)
Biosafety Level: 2
Availability Status: In Stock
Contributor: T Honda, M Yoh
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Escherichia coli (E. coli) (EHEC) strain RIMD 0509952 was isolated from human feces in 1996.

The enterohemorrhagic Escherichia coli (E. coli; EHEC) strain RIMD 0509952 was isolated from a patient during an outbreak of hemorrhagic colitis and hemolytic uremic syndrome in Sakai City, Osaka Prefecture, Japan. E. coli, RIMD 0509952 and many other EHEC strains encode potent toxins, similar to those of Shigella dysenteriae, which can cause severe intestinal, kidney and central nervous system disease. E. coli, RIMD 0509952 carries two plasmid species: 1) pO157 (92,721 bp) and 2) pOSAK1 (3,306 bp). Virulence-associated genes are located on both the chromosome and pO157. The complete sequence of the chromosome (5,498,450 bp; GenBank: NC_002695), pO157 (GenBank: NC_002128) and pOSAK1 (GenBank: NC_002127) from E. coli strain RIMD 0509952 have been determined.

Each vial contains approximately 0.5 mL of bacterial culture in 0.5X Tryptic Soy Broth supplemented with 10% glycerol.

Note: If homogeneity is required for your intended use, please purify prior to initiating work.
Citations: Acknowledgment for publications should read "The following reagent was obtained through the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH: Escherichia coli, Strain RIMD 0509952, NR-12."
Publications Citing
this Reagent:
Wang, F. "Development and Application of Serogroup-Independent and Serogroup-Specific Loop-Mediated Isothermal Amplification Assays for Detecting Shiga Toxin-Producing Escherichia coli." Diss. China Agricultural University, 2011.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
NR-2649
Derived from:  ATCC BAA-460
Genomic DNA from Escherichia coli, Strain RIMD 0509952
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