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BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-14815  Brucella abortus, 2308 ΔilvC
(Bacteria)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Brucella abortus
Designations: 2308 ΔilvC
Biosafety Level: 3
Availability Status: In Stock
Store at: -60°C or colder
Contributor: E Paramithiotis
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Brucella abortus (B. abortus), strain 2308 ΔilvC was generated from strain 2308 by deletion of the ilvC gene and replacement with a marker gene encoding resistance to kanamycin. B. abortus, strain 2308 is a well-studied standard laboratory biovar 1 strain, isolated in the USA, that is known to be virulent for humans, cattle and other domestic animals.

The ilvC protein is a ketol-acid reductoisomerase involved in the biosynthesis of leucine, isoleucine and valine [locus tag: BAB_RS22610 (previously, locus tag: BAB1_1399)]. The ilvC deletion in B. abortus, strain 2308, resulted in the attenuation of the strain for infection of murine macrophages (RAW264.7) which was determined by the contributor through an intracellular replication assay. A reduction in virulence has also been noted for a B. melitensisΔilvC strain. The complete genome sequence of B. abortus, strain 2308 is available (GenBank: JMRZ00000000).

Each vial contains approximately 0.7 mL of bacterial culture in Tryptic Soy broth supplemented with 10% glycerol.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Brucella abortus, Strain 2308 ΔilvC, NR-14815."
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
  • Select Agents require facility registration for specific agents with CDC and completion of APHIS/CDC Form 2. For information about toxin amounts excluded from CDC Select Agent regulations, click here.

RELATED PRODUCTS
NR-14816
Brucella abortus 2308 ΔwbkA
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