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BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-411  Bacillus anthracis, Ames (A0462)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Bacillus anthracis
Designations: Ames (A0462)
Biosafety Level: 3
Availability Status: In Stock
Contributor: P Keim
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Bacillus anthracis (B. anthracis), strain Ames (A0462, Asc 159) was isolated from a dead cow in Jim Hogg County, Texas in 1981. The organism was sent to USAMRIID where it was passaged in a guinea pig and mistakenly given the name "Ames". In the mid-1980s B. anthracis, strain Ames was provided to the Chemical and Biological Defense Sector and the Centre for Applied Microbiology and Research (CAMR) at Porton Down, Salisbury, UK. Professor Paul Keim received the bacteria from CAMR.

The presence of pXO1 and pXO2 in NR-411 has been confirmed by PCR amplification of plasmid-specific sequences from extracted DNA.

Each vial contains approximately 0.5 mL of bacterial culture in 0.5X Tryptic Soy Broth supplemented with 10% glycerol.

Additional information and tools are available at PATRIC (Pathosystems Resource Integration Center).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Bacillus anthracis, Strain Ames (A0462), NR-411."
Publications Citing
this Reagent:
Barrow, E. W., et al. "High-Throughput Screening of a Diversity Collection Using Biodefense Category A and B Priority Pathogens." J. Biomolec. Screen. 17 (2012): 946-956. PubMed: 22653912.

Dey, R., et al. "Germination and Amplification of Anthrax Spores by Soil-Dwelling Amoebas." Appl. Environ. Microbiol. 78 (2012): 8075-8081. PubMed: 22983962.

Riojas, M.A., et al. "Multiplex PCR for Species-Level Identification of Bacillus anthracis and Detection of pXO1, pXO2, and Related Plasmids." Health Secur. 13 (2015): 122-129. PubMed: 25813976.

Su, S., et al. "Construction and Characterization of Stable, Constitutively Expressed, Chromosomal Green and Red Fluorescent Transcriptional Fusions in the Select Agents, Bacillus anthracis, Yersinia pestis, Burkholderia mallei, and Burkholderia pseudomallei." Microbiol. Open (2014). PubMed: 25044501.

Su, S., et al. "Characterization of Stable, Constitutively Expressed, Chromosomal Green and Red Fluorescent Transcriptional Fusions in the Select Agent bacterium, Francisella tularensis Schu S4 and the Surrogate Type B Live Vaccine Strain (LVS)." Appl. Microbiol. Biotech 97 (2013): 9029-9041. PubMed: 23852642.
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
  • Select Agents require facility registration for specific agents with CDC and completion of APHIS/CDC Form 2. For information about toxin amounts excluded from CDC Select Agent regulations, click here.

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