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NR-45913  Staphylococcus aureus, RN4220/pG0400
(Bacteria)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Staphylococcus aureus
Designations: RN4220/pG0400
Biosafety Level: 2
Availability Status: In Stock
Store at: -60°C or colder
Contributor: GL Archer
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Staphylococcus aureus (S. aureus), strain RN4220/pG0400 (also referred to as NRS107) contains the plasmid pG0400 and is a transconjugant of mating between S. aureus, strain G03221 containing the plasmid pG0400 and S. aureus, strain RN4220NR. Strain G03221 was isolated in 1991 during an outbreak of mupirocin-resistant S. aureus on a dermatology ward of a university hospital in Connecticut, USA.

Plasmid pG0400 is a 33.8 kilobase plasmid that encodes resistance to mupirocin. S. aureus, strain RN4220/pG0400 is a methicillin-sensitive S. aureus (MSSA) strain. It was deposited as containing the plasmid pG0400; resistant to mupirocin, rifampicin and novobiocin; negative for mec; MLST sequence type (ST) 8; eGenomic spa type 59, eGenomic spa repeats YHGGFMBQBLO; Ridom spa type t211.

Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth supplemented with 10% glycerol.

Note: If homogeneity is required for your intended use, please purify prior to initiating work.
Citations: Acknowledgment for publications should read "The following reagent was provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) for distribution by BEI Resources, NIAID, NIH: Staphylococcus aureus, Strain RN4220/pG0400, NR-45913."
Additional Information: NARSA NRS107
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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