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NR-46412  Staphylococcus aureus, HIP13170
(Bacteria)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Staphylococcus aureus
Designations: HIP13170
Biosafety Level: 2
Availability Status: In Stock
Store at: -60°C or colder
Contributor: L Weigel
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Staphylococcus aureus (S. aureus), strain HIP13170 (also referred to as VRSA-3a and VRS3a) was isolated in 2004 in New York, USA, from urine of a 63-year-old female with a polymicrobial infected nephrostomy tube exit site who had no recent history of vancomycin therapy. It was co-isolated with S. aureus, strain HIP13419 (VSR3b) which are similar in most aspects except the vancomycin resistant phenotype for HIP13170 is less stable than that of HIP13419.

S. aureus, strain HIP13170 is a vancomycin-resistant S. aureus (VRSA) strain. S. aureus, strain HIP13170 was deposited as positive for mec (subtype IV), vanA and resistance genes against tetracycline, macrolides, lincosamides and aminoglycosides; negative for vanB, vanC1, vanC2, vanD and vanE; pulsed-field type USA800; MLST (ST) 5; spa repeats TJMBMDMGMK; Ridom spa type t002. This strain was isolated from the third documented case of VRSA infection in the United States. Unlike the other VRSA strains, it is believed that this strain obtained vanA from Enterococcus faecium rather than Enterococcus faecalis. S. aureus, strain HIP13170 is a USA800/Pediatric isolate. USA800 isolates have the same MLST profile (ST 5), agr group (II), SCCmec subtype (IV), spa motif (MDMGMK) and Ridom spa types (t002 and related) and are positive for sem and seo toxin genes. USA800 isolates are resistant to β-lactams with some isolates being resistant to additional antibiotics. While first isolated in pediatric patients, USA800 strains recently have been isolated in adults. The complete genome sequence of S. aureus, strain HIP13170 is available (GenBank: AHBM00000000).

Each vial contains approximately 0.5 mL of bacterial culture in Brain Heart Infusion broth supplemented with 6 µg/mL vancomycin and 10% glycerol.

Note: If homogeneity is required for your intended use, please purify prior to initiating work.
Citations: Acknowledgment for publications should read "The following reagent was provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) for distribution by BEI Resources, NIAID, NIH: Staphylococcus aureus, Strain HIP13170, NR-46412."
Additional Information: NARSA VRS3a
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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