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NR-46414  Staphylococcus aureus, HIP14300
(Bacteria)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Staphylococcus aureus
Designations: HIP14300
Biosafety Level: 2
Availability Status: In Stock
Store at: -60°C or colder
Contributor: J Patel
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Staphylococcus aureus (S. aureus), strain HIP14300 (also referred to as VRSA-4 and VRS4) was isolated in 2005 in Michigan, USA from a gangrenous toe wound of a 78-year-old male nursing home resident who was recently treated with a nine-week course of vancomycin.

S. aureus, strain HIP14300 is a vancomycin-resistant S. aureus (VRSA) strain. S. aureus, strain HIP14300 was deposited as positive for mec (subtype II) and vanA; negative for vanB, vanC1, vanC2, vanD, vanE, PVL and arginine catabolic mobile element (ACME); pulsed-field type USA100; spa repeats TJMBMDMGMK; Ridom spa type t002. S. aureus, strain HIP14300 is a USA100 isolate. USA100 isolates have the same MLST profile (ST 5), SCCmec (subtype II) and spa motif (MDMGMK) and are usually resistant to erythromycin and spectinomycin as well as being multiresistant to other commonly used therapeutic agents. USA100 is the most prevalent U.S health care-associated pulsed-field type and is endemic in many U.S. hospitals. The complete genome sequence of S. aureus, strain HIP14300 is available (GenBank: AHBN00000000).

Each vial contains approximately 0.5 mL of bacterial culture in Brain Heart Infusion broth supplemented with 6 µg/mL vancomycin and 10% glycerol.

Note: If homogeneity is required for your intended use, please purify prior to initiating work.
Citations: Acknowledgment for publications should read "The following reagent was provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) for distribution by BEI Resources, NIAID, NIH: Staphylococcus aureus, Strain HIP14300, NR-46414."
Additional Information: NARSA VRS4
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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