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NR-46421  Staphylococcus aureus, AIS 1001095
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Organism: Staphylococcus aureus
Designations: AIS 1001095
Biosafety Level: 2
Availability Status: In Stock
Store at: -60°C or colder
Contributor: B Limbago
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Staphylococcus aureus (S. aureus), strain AIS1001095 (also referred to as VRSA-11a and VRS11a) was isolated in 2010 in Delaware, USA from wound drainage of a 63-year-old female with a prosthetic joint infection who had been unsuccessfully treated with continuous vancomycin therapy for 3 months. Strain AIS 1001095 is a co-isolate with strain AID 1001123 (VRS11b) from the same patient and they were isolated at the same time. Both are mecA positive but unlike strain AID 1001123, strain AIS 1001095 is phenotypically susceptible to oxacillin by the cefoxitin disk diffusion test.

S. aureus, strain AIS 1001095 is a vancomycin-resistant S. aureus (VRSA) strain. S. aureus, strain AIS 1001095 was deposited as positive for mec and vanA; negative for vanB, PVL and arginine catabolic mobile element (ACME); pulsed-field type USA100; spa repeats TJMBMDMGMK; Ridom spa type t002. S. aureus, strain AIS 1001095 is a USA100 isolate. USA100 isolates have the same MLST profile (ST 5), SCCmec (subtype II) and spa motif (MDMGMK) and are usually resistant to erythromycin and spectinomycin as well as being multiresistant to other commonly used therapeutic agents. USA100 is the most prevalent U.S health care-associated pulse-field type and is endemic in many U.S. hospitals. S. aureus, strain AIS 1001095 is partially dependent on vancomycin for growth due to a mutation in D-alanyl:D-alanine ligase (Ddl), an enzyme involved in biosynthesis of peptidoglycans. The presence of the vanA operon compensates for the Ddl mutation by providing alternative peptidoglycans for cell wall synthesis; however, some have found that the change in the type of peptidoglycans produced renders it susceptible to oxacillin. The complete genome sequence of S. aureus, strain AIS 1001095 is available (GenBank: AHBU00000000).

Each vial contains approximately 0.5 mL of bacterial culture in Brain Heart Infusion broth supplemented with 6 µg/mL vancomycin and 10% glycerol.
Citations: Acknowledgment for publications should read "The following reagent was provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) for distribution by BEI Resources, NIAID, NIH: Staphylococcus aureus, Strain AIS 1001095, NR-46421."
Additional Information: NARSA VRS11a
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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