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NR-97  Escherichia coli, H414-36/89 (Serotype O91:H21)
(Bacteria)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Escherichia coli
Designations: H414-36/89 (Serotype O91:H21)
Biosafety Level: 2
Availability Status: In Stock
Store at: -60°C
Contributor: AD O'Brien, JF Sinclair
Comments:
E. coli is a gram-negative, rod-shaped bacterium which occurs singly or in pairs. It is a major facultative inhabitant of the large intestine.

The enterohemorrhagic E. coli (EHEC) strain H414-36/89 was isolated from a patient with hemorrhagic colitis in Germany. E. coli H414-36/89 and many other EHEC strains encode potent toxins, similar to those of Shigella dysenteriae, which can cause severe intestinal, kidney and central nervous system disease. The large plasmid of E. coli H414-36/89 carries one copy of the gene for Shiga-like toxin type II (SLT-II) and two copies of the gene for an SLT-II-variant-type toxin. E. coli H414 36/89 has been shown to be highly virulent in a mouse model of infection.

Each vial contains approximately 0.5 mL of bacterial culture in 0.5X Tryptic Soy Broth supplemented with 10% glycerol.

Note: If homogeneity is required for your intended use, please purify prior to initiating work.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Escherichia coli, Strain H414-36/89, NR-97."
Publications Citing
this Reagent:
Wang, F. "Development and Application of Serogroup-independent and Serogroup-specific Loop-mediated Isothermal Amplification Assays for Detecting Shiga Toxin-Producing Escherichia coli." Diss. China Agricultural University, 2011.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
NR-2650
Derived from:  ATCC 51434
Genomic DNA from Escherichia coli, Strain H414-36/89
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