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-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
ARP-502 is derived from the parental HeLa cell line transfected with a Moloney-based retroviral vector containing a SalI-BamHI segment from the human immunodeficiency virus 1 (HIV-1) genomic clone pHXBc2 and a neomycin selection marker. Clones were selected for neomycin-resistance and screened for their ability to trans-activate a transfected HIV-1 long terminal repeat (LTR). Because expression of the tat protein is stable, the cells can be grown for weeks in the absence of neomycin. If expression drops, the cells can be grown in the presence of 800 µg per mL neomycin.
Growth Characteristics: ARP-502 is an adherent cell line that grows to a confluent culture in 3 to 4 days. Split cultures at a ratio of 1:10. Horse serum is optional for cultivating the cells. They can be maintained as adherent cultures in 10% fetal bovine serum. Horse serum is necessary to maintain the cells as suspension cultures; however, once the cells are grown in FBS they cannot be easily grown in horse serum again.
Growth Medium: DMEM supplemented with 7% horse serum
ARP-502 is negative for bacteria, Mycoplasma, yeast and mold.
Each vial of ARP-502 contains approximately 5 × 106 cells in DMEM supplemented with 40% fetal bovine serum and 10% dimethyl sulfoxide (DMSO). Please refer to the attached file(s) for additional information.
Rosen, C. A., et al. “Construction of Recombinant Murine Retroviruses that Express the Human T Cell Leukemia Virus Type II and Human T Cell Lymphotropic Virus Type III Trans-Activator Genes.” J. Virol. 57 (1985): 379-384.PubMed: 3001360.
Terwilliger, E., et al. “Cell Lines that Express Stable env Gene Products from Three Strains of HIV-1.” J. Acquir. Immune Defic. Syndr. 1 (1988): 317-323. PubMed: 3265154.
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