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MRA-100   Hybridoma 3D11 Anti-Plasmodium berghei 44-Kilodalton Sporozoite Surface Protein (Pb44)
(Cell Banks)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Hybridoma 3D11 Anti-Plasmodium berghei 44-Kilodalton Sporozoite Surface Protein (Pb44)
Organism: Plasmodium berghei
Biosafety Level: 1
Availability Status: In Stock
Store at: -100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Contributor: V Nussenzweig
Comments:
Quantity limit per order for this item is 1. This item can be ordered once a year. Orders over this limit will be sent to NIAID for approval before shipment.

The murine hybridoma cell line, 3D11, was generated by the fusion of P3UI mouse myeloma cells with splenocytes from BALB/c mice immunized by exposure to repeated bites of gamma-irradiated Plasmodium berghei (P. berghei)-infected mosquitoes. This reagent was authenticated by the contributor and tested for mycoplasma.

Each vial of MRA-100 contains approximately 1 mL of unpurified cell culture suspension frozen in medium containing cryopreservative.

The monoclonal antibody is specific for P. berghei sporozoites and can neutralize sporozoites in vitro. The antibody binds to a P. berghei 44 kDa membrane protein (Pb44) on the sporozoite surface.

Yoshida, N., et al. "Hybridoma Produces Protective Antibodies Directed Against the Sporozoite Stage of Malaria Parasite." Science 207 (1980): 71-73. PubMed: 6985745.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Hybridoma 3D11 Anti-Plasmodium berghei 44-Kilodalton Sporozoite Surface Protein (Pb44), MRA-100, contributed by Victor Nussenzweig."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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