| |
|
Product Name:
|
Hybridoma 2A10 Anti-Plasmodium falciparum Circumsporozoite Protein (CSP)
|
|
Manufacturer:
|
BEI Resources
|
|
Material Provided:
|
Each vial contains approximately 0.5 to 1 mL of hybridoma cells in cell culture medium supplemented with 10% dimethylsulfoxide (DMSO) at a concentration of approximately 107 cells per mL. Please see Appendix I for media preparation. Sufficient cells are provided to initiate at least one new culture.
|
|
Packing/Storage:
|
This product was packaged aseptically in cryovials. It should be stored at -100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer. Storage at -70°C will result in loss of viability. To insure the highest level of viability, the vial should be thawed and the culture initiated as soon as possible upon receipt. Any warming of the product during shipping and transfer must be avoided, as this will adversely affect the viability of the product after thawing. For transfer between freezers and shipping, the cells may be placed on dry ice for brief periods, although use of a portable liquid nitrogen carrier is preferred. Please read the following recommendations prior to reconstituting this material.
|
|
Safety Precautions:
|
When handling frozen vials it is highly recommended that protective gloves, lab coat and full face mask be worn. Even brief exposure to the ultra-cold temperature can cause tissue damage from frostbite. Also, some vials may slowly fill with liquid nitrogen if they have been immersed during cryogenic storage. When thawing, the liquid nitrogen may rapidly expand as it changes to gas, breaking the vial or cap with explosive force, sending debris flying with enough velocity to cause injury. Store and use in areas with adequate ventilation. Subcultivation Procedure: Prior to thawing the hybridoma cells, prepare cell culture medium according to Appendix I. Thaw one vial in a 37°C water bath and transfer the contents into a 25 cm cell culture flask with 10 mL of cell culture medium. Keep the flask loosely capped in a 37°C incubator with 5% CO2. Change media at 12-16 hours post-seeding. Feed cells at least every 48 hours and split cells when 70% confluent. Citation: Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Hybridoma 2A10 Anti-Plasmodium falciparum Circumsporozoite Protein (CSP), MRA-183, contributed by Elizabeth Nardin.” Biosafety Level: 1 Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories. 5th ed. Washington, DC: U.S. Government Printing Office, 2009; see www.cdc.gov/biosafety/publications/bmbl5/index.htm. Disclaimers: You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. BEI Resources E-mail: contact@beiresources.org www.beiresources.org Tel: 800-359-7370 Fax: 703-365-2898 © 2019/2020 American Type Culture Collection (ATCC). All rights reserved. MRA-183_15JUL2020 Page 1 of 2 Product Information Sheet for MRA-183 Use Restrictions: This material is distributed for internal research, non?commercial purposes only. This material, its product or its derivatives may not be distributed to third parties. Except as performed under a U.S. Government contract, individuals contemplating commercial use of the material, its products or its derivatives must contact the contributor to determine if a license is required. U.S. Government contractors may need a license before first commercial sale. References: 1. Nardin, E., Personal Communication. 2. Zavala, F., et al. “Circumsporozoite Proteins of Malaria Parasites Contain a Single Immunodominant Region with Two or More Identical Epitopes.” J. Exp. Med. 157 (1983): 1947-1957. PubMed: 6189951. 3. Hollingdale, M. R., et al. “Inhibition of Entry of Plasmodium falciparum and P. vivax Sporozoites into Cultured Cells; an in vitro Assay of Protective Antibodies.” J. Immunol. 132 (1984): 909-913. PubMed: 6317752. 4. Zavala, F., et al. “Rationale for Development of a Synthetic Vaccine against Plasmodium falciparum Malaria.” Science 228 (1985): 1436-1440. PubMed: 2409595. 5. Wirtz, R. A., et al. “Comparative Testing of Monoclonal Antibodies against Plasmodium falciparum Sporozoites for ELISA Development.” Bull. World Health Organ. 65 (1987): 39-45. PubMed: 3555879. 6. Zhang, M., et al. “Monoclonal Antibodies against Plasmodium falciparum Circumsporozoite Protein.” Antibodies 6 (2017): 11. 7. Nardin, E., et al. “Circumsporozoite Proteins of Human Malaria Parasites Plasmodium falciparum and Plasmodium vivax.” J. Exp. Med. 156 (1982): 20-30. PubMed: 7045272. ATCC® is a trademark of the American Type Culture Collection. APPENDIX I: MEDIA PREPARATION Cell Culture Medium Advanced RPMI 1640 medium (Gibco™ 12633; 1×) Supplemented with: Fetal Bovine Serum (FBS, hybridoma-tested; 10%) L-glutamine (4 mM) Gentamicin (optional; 50 µg per mL) Freezing Medium Cell culture medium (as above) 10% DMSO Freeze at 107 cells per mL BEI Resources E-mail: contact@beiresources.org www.beiresources.org Tel: 800-359-7370 Fax: 703-365-2898 © 2019/2020 American Type Culture Collection (ATCC). All rights reserved. MRA-183_15JUL2020 Page 2 of 2
|
|
Functional Activity:
|
Hybridoma 2A10 produces monoclonal antibody of the IgG2aκ subclass, which is specific for P. falciparum CSP repeats, and is reported to function in ELISA, immunofluorescence, immunoprecipitation, electron microscopy and immunoblot assays. 1,2,3
|
|
Disclaimers:
|
You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
|
1. Nardin, E., Personal Communication. 2. Zavala, F., et al. “Circumsporozoite Proteins of Malaria Parasites Contain a Single Immunodominant Region with Two or More Identical Epitopes.” J. Exp. Med. 157 (1983): 1947-1957. PubMed: 6189951. 3. Hollingdale, M. R., et al. “Inhibition of Entry of Plasmodium falciparum and P. vivax Sporozoites into Cultured Cells; an in vitro Assay of Protective Antibodies.” J. Immunol. 132 (1984): 909-913. PubMed: 6317752. 4. Zavala, F., et al. “Rationale for Development of a Synthetic Vaccine against Plasmodium falciparum Malaria.” Science 228 (1985): 1436-1440. PubMed: 2409595. 5. Wirtz, R. A., et al. “Comparative Testing of Monoclonal Antibodies against Plasmodium falciparum Sporozoites for ELISA Development.” Bull. World Health Organ. 65 (1987): 39-45. PubMed: 3555879. 6. Zhang, M., et al. “Monoclonal Antibodies against Plasmodium falciparum Circumsporozoite Protein.” Antibodies 6 (2017): 11. 7. Nardin, E., et al. “Circumsporozoite Proteins of Human Malaria Parasites Plasmodium falciparum and Plasmodium vivax.” J. Exp. Med. 156 (1982): 20-30. PubMed: 7045272.
|
|
Citation:
|
Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Hybridoma 2A10 Anti-Plasmodium falciparum Circumsporozoite Protein (CSP), MRA-183, contributed by Elizabeth Nardin.”
|
|
Biosafety Level:
|
1
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
|