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The murine hybridoma cell line, 6D12, was generated by the fusion of SP2/0 mouse myeloma cells with splenocytes from BALB/c mice immunized with salivary gland membranes of Aedes aegypti (Ae. aegypti), strain Black-Eye Liverpool mosquitoes. The monoclonal antibody produced binds preferentially to the distal lateral lobes of female and male Ae. aegypti salivary
glands. The 6D12 antibody does not cross-react with salivary glands of Anopheles gambiae and Culex pipiens mosquitoes.
Hybridoma 6D12 produces monoclonal antibody of the IgG1 subclass, which is specific for female and male Ae. aegypti salivary glands, and is reported to function in immunoblot and immunofluorescence assays.
Each vial contains approximately 0.5 mL of hybridoma cells in cell culture medium supplemented with 10% dimethylsulfoxide (DMSO) at a concentration of 106 cells per mL. Sufficient cells are provided to initiate at least one new culture.
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