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MRA-260   Hybridoma 18E12 Anti-Aedes aegypti Salivary Glands
(Cell Banks)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Hybridoma 18E12 Anti-Aedes aegypti Salivary Glands
Organism: Aedes aegypti
Biosafety Level: 1
Availability Status: In Stock
Store at: -100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Contributor: KD Vernick
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The murine hybridoma cell line, 18E12, was generated by the fusion of SP2/0 mouse myeloma cells with splenocytes from BALB/c mice immunized with salivary gland membranes of Aedes aegypti (Ae. aegypti), strain Black-Eye Liverpool mosquitoes. The monoclonal antibody produced binds preferentially to the distal lateral lobes of female and male Ae. aegypti salivary glands; it also recognizes ovarian tissue. The 18E12 antibody does not cross-react with salivary glands of Anopheles gambiae but does with those of Culex pipiens mosquitoes.

Hybridoma 18E12 produces monoclonal antibody of the IgG1 subclass, which is specific for female and male Ae. aegypti salivary glands, and is reported to function in immunoblot and immunofluorescence assays.

Each vial contains approximately 0.5 mL of hybridoma cells in cell culture medium supplemented with 10% dimethylsulfoxide (DMSO) at a concentration of 106 cells per mL. Sufficient cells are provided to initiate at least one new culture.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Hybridoma 18E12 Anti-Aedes aegypti Salivary Glands, MRA-260, contributed by Kenneth D. Vernick."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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