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MRA-315   Hybridoma 4B7 Anti-Plasmodium falciparum 25-Kilodalton Gamete Surface Protein (Pfs25)
(Cell Banks)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Hybridoma 4B7 Anti-Plasmodium falciparum 25-Kilodalton Gamete Surface Protein (Pfs25)
Organism: Plasmodium falciparum
Biosafety Level: 1
Availability Status: In Stock
Store at: -100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Contributor: LH Miller, A Saul
Comments:
Quantity limit per order for this item is 1. This item can be ordered once a year. Orders over this limit will be sent to NIAID for approval before shipment.

The murine hybridoma cell line, 4B7, was generated by the fusion of mouse myeloma cells with splenocytes from mice immunized twice with recombinant vaccinia virus expressing the 25-kDa gamete surface protein of Plasmodium falciparum (P. falciparum) (Pfs25), and boosted with whole P. falciparum gametes. This reagent was authenticated by the contributor and tested for mycoplasma.

Each vial of MRA-315 contains approximately 0.5 mL of hybridoma cells (5 × 106 cells/mL) in cell culture medium supplemented with 10% dimethylsulfoxide (DMSO).

Hybridoma 4B7 produces monoclonal antibody that specifically recognizes Pfs25 with no known cross reactivity.

Barr, P. J., et al. "Recombinant Pfs25 Protein of Plasmodium falciparum Elicits Malaria Transmission-Blocking Immunity in Experimental Animals." J. Exp. Med. 174 (1991): 1203-1208. PubMed: 1940798.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Hybridoma 4B7 Anti-Plasmodium falciparum 25-Kilodalton Gamete Surface Protein (Pfs25), MRA-315, contributed by Louis H. Miller and Allan Saul."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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