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MRA-658   Hybridoma 13.5 Anti-Plasmodium chabaudi Erythrocyte Membrane Protein [Pc(em)-93]
(Cell Banks)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Hybridoma 13.5 Anti-Plasmodium chabaudi Erythrocyte Membrane Protein [Pc(em)-93]
Organism: Plasmodium chabaudi
Biosafety Level: 1
Availability Status: In Stock
Store at: -100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Contributor: MF Wiser
Quantity limit per order for this item is 1. This item can be ordered once a year. Orders over this limit will be sent to NIAID for approval before shipment.

The murine hybridoma cell line, 13.5, was generated by the fusion of mouse myeloma cells (P3X63.Ag8.6.5.3) with splenocytes from mice immunized with a purified, glutamate-rich, 93-kDa erytrocyte-membrane-associated protein from Plasmodium chabaudi (P. chabaudi), Pc(em)-93. This reagent was authenticated by the contributor and tested for mycoplasma.

Each vial of MRA-658 contains approximately 0.5 mL of hybridoma cells (5 × 106 cells/mL) in cell culture medium supplemented with 10% dimethylsulfoxide (DMSO).

Hybridoma 13.5 produces monoclonal antibody (isotype IgG1) that specifically recognizes an approximately 90-kDa P. chabaudi phosphoprotein associated with the erytrocyte membrane. The antigen recognized by the 13.5 antibody is an excellent marker for P. chabaudi-infected erythrocyte membranes and has been used to study protein export in infected erythrocytes.

Wiser, M. F., M. B. Leible and B. Plitt. "Acidic Phosphoproteins Associated with the Host Erythrocyte Membrane of Erythrocytes Infected with Plasmodium berghei and P. chabaudi." Mol. Biochem. Parasitol. 27 (1988): 11-22. PubMed: 3278220.
Wiser, M. F., et al. "Plasmodium chabaudi: Immunogenicity of a Highly Antigenic Glutamate-Rich Protein." Exp. Parasitol. 85 (1997): 43-54. PubMed: 9024201.
Giraldo, L. E., et al. "Molecular Characterization of a Plasmodium chabaudi Erythrocyte Membrane-Associated Protein with Glutamate-Rich Tandem Repeats." J. Eukaryot. Microbiol. 45 (1998): 528-534. PubMed: 9783454.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Hybridoma 13.5 Anti-Plasmodium chabaudi Erythrocyte Membrane Protein [Pc(em)-93], MRA-658, contributed by Mark F. Wiser."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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