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MRA-661   Hybridoma 17.4 Anti-Plasmodium berghei Erythrocyte Membrane Protein Pb(em)-46
(Cell Banks)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Hybridoma 17.4 Anti-Plasmodium berghei Erythrocyte Membrane Protein Pb(em)-46
Organism: Plasmodium berghei
Biosafety Level: 1
Availability Status: In Stock
Store at: -100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Contributor: MF Wiser
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The murine hybridoma cell line, 17.4, was generated by the fusion of X63Ag8.653 mouse myeloma cells with splenocytes from mice immunized with purified Plasmodium berghei (P. berghei) erythrocyte membrane protein, Pb(em)-46. Pb(em)-46 is a 46-kDa phosphoprotein associated with the submembrane cytoskeleton of P. berghei-infected erythrocytes. The original hybridoma was described as 17.2; hybridoma 17.4 produces a monoclonal antibody of the same specificity, but has better growth characteristics.

Hybridoma 17.4 produces monoclonal antibody of IgG1 subclass that specifically recognizes an approximately 46-kDa phosphoprotein found on the P. berghei-infected erythrocyte membrane. The antibody is reported to function in immunoblotting and immunofluorescence assays.

Each vial contains approximately 0.5 mL of hybridoma cells in cell culture medium supplemented with 10% dimethylsulfoxide (DMSO) at a concentration of 107 cells per mL. Sufficient cells are provided to initiate at least one new culture.
Citations: Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Hybridoma 17.4 Anti-Plasmodium berghei Erythrocyte Membrane Protein Pb(em)-46, MRA-661, contributed by Mark F. Wiser.”
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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