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MRA-669   Hybridoma W3.5 Anti-Plasmodium berghei Erythrocytic-Stage Exoantigen
(Cell Banks)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Hybridoma W3.5 Anti-Plasmodium berghei Erythrocytic-Stage Exoantigen
Organism: Plasmodium berghei
Biosafety Level: 1
Availability Status: In Stock
Store at: -100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Contributor: MF Wiser
Comments:
Quantity limit per order for this item is 1. This item can be ordered once a year. Orders over this limit will be sent to NIAID for approval before shipment.

The murine hybridoma cell line, W3.5, was generated by the fusion of mouse myeloma cells (X63.Ag8.6.5.3) with splenocytes from immunized mice, which were infected with Plasmodium berghei (P. berghei) and cured with chloroquine twice. This reagent was authenticated by the contributor and tested for mycoplasma.

Each vial of MRA-669 contains approximately 0.5 mL of hybridoma cells (5 × 106 cells/mL) in cell culture medium supplemented with 10% dimethylsulfoxide (DMSO).

Hybridoma W3.5 produces IgG2b antibody that specifically recognizes an unknown 31 kDa protein of Plasmodium berghei and an unidentified 13 kDa to 18 kDa protein associated with inclusions in the cytoplasm of erythrocytes infected with rodent Plasmodium species. Applications include Western Blot, immunoprecipitation and immunofluorescence (produced external to the parasite and within the host erythrocyte cytoplasm).

Wiser, M. F. "Characterization of Monoclonal Antibodies Directed against Erythrocytic Stage Antigens of Plasmodium berghei." Eur. J. Cell Biol. 42 (1986): 45-51. PubMed: 3539609.
Wiser, M. F. "Plasmodium Antigens External to the Parasite But with the Infected Erythrocyte." Parasitol. Res. 75 (1989): 206-211. PubMed: 2652137.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Hybridoma W3.5 Anti-Plasmodium berghei Erythrocytic-Stage Exoantigen, MRA-669, contributed by Mark F. Wiser."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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