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BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
MRA-896   Hybridoma 58F8 Anti-Plasmodium falciparum 83-kDa Apical Membrane Antigen 1 (PF83/AMA-1)
(Cell Banks)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Hybridoma 58F8 Anti-Plasmodium falciparum 83-kDa Apical Membrane Antigen 1 (PF83/AMA-1)
Organism: Plasmodium falciparum
Biosafety Level: 1
Availability Status: In Stock
Store at: -100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Contributor: AW Thomas
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The murine hybridoma cell line, 58F8, was generated by the fusion of rat Y3-Ag 1.2.3 myeloma cells with splenocytes from LOU/M rats immunized with a synthetic peptide. The antibody produced binds preferentially to an N-terminal peptide of Plasmodium falciparum 83-kDa apical membrane antigen 1 (PF83/AMA-1).

Hybridoma 58F8 produces monoclonal antibody of IgG2a subclass and binds preferentially to an N-terminal peptide of PF83/AMA-1. The antibody is reported to function in ELISA, western blot, immunofluorescence and immunoprecipitation assays.

Each vial contains approximately 0.5 mL of hybridoma cells in cell culture medium supplemented with 10% dimethylsulfoxide (DMSO) at a concentration of 107 cells per mL. Sufficient cells are provided to initiate at least one new culture.
Citations: Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Hybridoma 58F8 Anti-Plasmodium falciparum 83-kDa Apical Membrane Antigen 1 (PF83/AMA-1), MRA-896, contributed by Alan W. Thomas.”
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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