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NR-10385  Cercopithecus aethiops, Vero, Kidney (African green monkey), Expressing Luciferase (Luc2p)
(Cell Banks)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Vero, Kidney (African green monkey), Expressing Luciferase (Luc2p)
Organism: Cercopithecus aethiops
Biosafety Level: 1
Availability Status: In Stock
Store at: -100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Contributor: AA Weiss
Comments:
This catalog item can be shipped to domestic locations within the United States only. International shipments are not available at this time. Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Vero cells are an adherent epithelial cell line derived from the kidney of an African green monkey (Cercopithecus aethiops). NR-10385 consists of Vero cells engineered to stably express the Luc2p variant of luciferase. Luc2p is tagged with a proline- glutamic acid-serine-threonine (PEST) sequence, which promotes rapid turnover by targeting the protein to the proteosome for degradation. Since measurable luciferase activity is due to newly synthesized protein, luciferase decreases when protein synthesis is inhibited. Vero cells expressing Luc2p have been used in the development of a quantitative and sensitive luciferase-based assay for bacterial toxins that inhibit protein synthesis.

Each vial contains approximately 1 mL of cell culture suspension frozen in cell growth medium (90%) and DMSO (10%) cryo-preservatant. Sufficient cells are provided to initiate at least one new culture. (Note: These cells are not recommended for use beyond passage 22 as they begin to lose sensitivity.)
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vero, Kidney (African green monkey), Expressing Luciferase (Luc2p), NR-10385."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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