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NR-17605  Vaccinia virus, Western Reserve Genome, VAC(WR)-LoxP-GFP-BAC, Recombinant in Escherichia coli
(Cell Banks)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Western Reserve Genome, VAC(WR)-LoxP-GFP-BAC, Recombinant in Escherichia coli
Organism: Vaccinia virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -60°C or colder
Contributor: B Moss
Comments:
Scientists at for-profit institutions must contact the National Institute of Allergy and Infectious Diseases (NIAID), Email: kstemple@niaid.nih.gov before the reagent can be released. Please specify the name, BEI catalog number and a brief description of the intended use of the reagent in your email to NIAID.

Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The entire vaccinia virus (VACV) Western Reserve (WR) genome (~195 Kb) (NCBI Accession AY243312) with a green fluorescent protein (GFP) sequence and two loxP sites was cloned as a bacterial artificial chromosome (BAC) and grown in Escherichia coli (E. coli) DH10ß cells harboring a mini-lambda prophage. The integrated mini-lambda encodes the red recombination system, comprising a 5' to 3' exonuclease (Exo), a single-strand DNA binding protein (Bet), and a nuclease inhibitor (Gam), under the control of the temperature-sensitive λ cI857 repressor. E. coli DH10β/VAC-BAC/λ provides a system to achieve recombination between the VAC-BAC atificial chromosome and electroporated linear DNA with homologous sequences to facilitate production of recombinant VACV expression vectors or mutagenesis of the VACV genome.

Each vial contains approximately 200 µL of NR-17605 in Luria-Bertani (LB) broth supplemented with 10% glycerol.

The presence of authentic VACV WR and GFP sequences in NR-17605 has been confirmed by PCR amplification and partial nucleotide sequencing. The sequence of the entire VACV WR genome has not been confirmed due to the large size of the plasmid insert. The presence of the integrated mini-lambda prophage also has not been confirmed at BEI Resources.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vaccinia Virus Western Reserve Genome, VAC(WR)-LoxP-GFP-BAC, Recombinant in Escherichia coli, NR-17605."
PERMITS
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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