Contact Us   Having technical issues or questions regarding this product?
Please Contact Us.
BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-17606  Vaccinia virus, Western Reserve Genome, VAC(WR)-LoxP-GFP-BAC/Zeo, Recombinant in Escherichia coli
(Cell Banks)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Western Reserve Genome, VAC(WR)-LoxP-GFP-BAC/Zeo, Recombinant in Escherichia coli
Organism: Vaccinia virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -60°C or colder
Contributor: B Moss
Scientists at for-profit institutions must contact the National Institute of Allergy and Infectious Diseases (NIAID), Email: before the reagent can be released. Please specify the name, BEI catalog number and a brief description of the intended use of the reagent in your email to NIAID.

Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The entire vaccinia virus (VACV) Western Reserve (WR) genome (~195 Kb) (NCBI Accession AY243312) with a green fluorescent protein (GFP) sequence, two loxP sites, and a zeomycin resistance gene (zeoR) was cloned as a bacterial artificial chromosome (BAC) and grown in Escherichia coli (E. coli) DH10ß cells harboring a mini-lambda prophage. The integrated mini-lambda encodes the red recombination system, comprising a 5' to 3' exonuclease (Exo), a single-strand DNA binding protein (Bet), and a nuclease inhibitor (Gam), under the control of the temperature-sensitive λ cI857 repressor. E. coli DH10β/VAC-BAC/λ provides a system to achieve recombination between the VAC-BAC artificial chromosome and electroporated linear DNA with homologous sequences to facilitate production of recombinant VACV expression vectors or mutagenesis of the VACV genome. The chloramphenicol acetyl transferase (cat) gene promoter in the VAC-BAC is inactivated by replacement with the zeoR gene, making NR-17606 chloramphenicol sensitive and zeomycin resistant. Subsequent reactivation by inclusion of a cat promoter in the recombination cassette allows selection of recombinant VAC-BAC based on chloramphenicol resistance.

Each vial contains approximately 200 µL of NR-17606 in Luria-Bertani (LB) broth supplemented with 10% glycerol.

The presence of authentic VACV WR and GFP sequences in NR-17606 has been confirmed by PCR amplification and partial nucleotide sequencing. The sequence of the entire VACV WR genome has not been confirmed due to the large size of the plasmid insert. The presence of the integrated mini-lambda prophage also has not been confirmed at BEI Resources.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vaccinia Virus, Western Reserve Genome, VAC(WR)-LoxP-GFP-BAC/Zeo, Recombinant in Escherichia coli, NR-17606."
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Return to Top

Notices and Disclaimers

BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.

Back to My Search