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NR-9459  Mus musculus, Macrophage Cell Line Derived from MAL Knockout Mice
(Cell Banks)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Macrophage Cell Line Derived from MAL Knockout Mice
Organism: Mus musculus
Biosafety Level: 2
Availability Status: In Stock
Store at: -100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Contributor: D Golenbock
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The murine macrophage cell line, NR-9459, was derived using primary bone marrow cells from MAL (MyD88 adaptor-like) knockout mice. The primary bone marrow cells were immortalized by infection with the ecotropic transforming replication-deficient retrovirus J2 using techniques described in the literature. Characterization based on immunofluorescence, stimulation assays and flow cytometry demonstrated that the immortalized cell line retains its macrophage-specific morphological, functional and surface expression properties.

Each vial contains approximately 1 mL of cell culture suspension frozen in cell growth medium (90%) and DMSO (10%) cryopreservative. Sufficient cells are provided to initiate at least one new culture.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Macrophage Cell Line Derived from MAL Knockout Mice, NR-9459."
Publications Citing
this Reagent:
Cuevas, C. D., et al. "Junin Virus Infects Mouse Cells and Induces Innate Immune Responses." J. Virol. 85 (2011): 11058-11068. PubMed: 21880772.

Hayes, M. W., et al. "Pathogenic Old World Arenaviruses Inhibit TLR2/Mal-Dependent Proinflammatory Cytokines In Vitro." J. Virol. 86 (2012): 7216-7226. PubMed: 22532679.

Powell, D. A. "Immune Responses to a Francisella Lipid A Mutant: Characterization and Therapeutic Potential." Diss. University of Maryland, Baltimore, 2012.
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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