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HM-782D  Mixed bacteria, Genomic DNA from Microbial Mock Community B (Even, Low Concentration), v5.1L, for 16S rRNA Gene Sequencing
(Other Products)
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at no cost to registered researchers.
Description: Genomic DNA from Microbial Mock Community B (Even, Low Concentration), v5.1L, for 16S rRNA Gene Sequencing
Organism: Mixed bacteria
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: SK Highlander
Quantity limit per order for this item is 1.  This item can be ordered once a year.  Orders over this limit will be sent to NIAID for approval before shipment.

HM-782D contains genomic DNA from 20 bacterial strains containing equimolar (Even) ribosomal RNA (rRNA) operon counts (100,000 copies per organism per µL). This mock community is recommended for 16S rRNA gene sequencing by Sanger or amplicon sequencing methods. The recommended amount to use per experiment is 1 µL.

HM-782D has been qualified for PCR applications by amplification of approximately 1500 base pairs of the 16S rRNA gene.

Each vial contains approximately 100 ng of the bacterial genomic DNA mixture suspended in 25 µL TE buffer (10 mM Tris-HCl and 1 mM EDTA, pH ~ 7.4).  Note: If the vial is dry, add 25 µLTE buffer and incubate overnight at 4°C with mixing to resolubilize the sample.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH as part of the Human Microbiome Project: Genomic DNA from Microbial Mock Community B (Even, Low Concentration), v5.1L, for 16S rRNA Gene Sequencing, HM-782D."
Publications Citing
this Reagent:
Pearce, M. M., et al. "The Female Urinary Microbiome: A Comparison of Women with and without Urgency Urinary Incontinence." mBio 75 (2014): e01283-01214. PubMed: 25006228.
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.

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