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HM-783D Mixed bacteria, Genomic DNA from Microbial Mock Community B (Staggered, Low Concentration), v5.2L, for 16S rRNA Gene Sequencing
(Other Products)

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at no cost to registered researchers.

Description:

Genomic DNA from Microbial Mock Community B (Staggered, Low Concentration), v5.2L, for 16S rRNA Gene Sequencing

Organism:

Mixed bacteria

Biosafety Level:

Availability Status:

In Stock

Store at:

-20°C or colder

Contributor:

SK Highlander

Comments:

Quantity limit per order for this item is 1. This item can be ordered once a year. Orders over this limit will be sent to NIAID for approval before shipment.

HM-783D contains genomic DNA mixture from 20 bacterial strains containing staggered ribosomal RNA (rRNA) operon counts (1,000 to 1,000,000 copies per organism per µL). This mock community is recommended for 16S rRNA gene sequencing by Sanger or amplicon sequencing methods. The recommended amount to use per experiment is 1 µL.

HM-783D has been qualified for PCR applications by amplification of approximately 1500 base pairs of the 16S ribosomal RNA gene.

Each vial contains approximately 35 µL of the bacterial genomic DNA mixture suspended in TE buffer (10 mM Tris-HCl and 1 mM EDTA, pH ~ 7.4).

Citations:

Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH as part of the Human Microbiome Project: Genomic DNA from Microbial Mock Community B (Staggered, Low Concentration), v5.2L, for 16S rRNA Gene Sequencing, HM-783D."

Publications Citing this Reagent:

Berkell, M., et al. “Microbiota-Based Markers Predictive of Development of Clostridioides difficile Infection.” Nat. Commun. 12 (2021): 2241. doi: 10.1038/s41467-021-22302-0. PubMed: 33854066.

Restrictions On Use:

This material is distributed for internal research, noncommercial purposes only. This material, its product or its derivatives may not be distributed to third parties. Except as performed under a U.S. Government contract, individuals contemplating commercial use of the material, its products or its derivatives must contact the contributor to negotiate a license. U.S. Government contractors may need a license before first commercial sale.

Product Name:

Genomic DNA from Microbial Mock Community B (Staggered, Low Concentration), v5.2L, for 16S rRNA Gene Sequencing

Disclaimers:

You are authorized to use this product for research use only. It is not intended for human use.

Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC^® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC^® nor the U.S. Government warrants that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC^® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC^®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products.

Citation:

Biosafety Level:

1 Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.

Product Information Sheet

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Notices and Disclaimers

BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.

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Product Highlights for HM-783D

Product Sheet Information for HM-783D Mixed bacteria, Genomic DNA from Microbial Mock Community B (Staggered, Low Concentration), v5.2L, for 16S rRNA Gene Sequencing, For research use only. Not for use in humans.

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