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NR-18958  Mycobacterium tuberculosis, DeADMAn DNA Microarray, 12K, Version 2
(Other Products)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: DeADMAn DNA Microarray, 12K, Version 2
Organism: Mycobacterium tuberculosis
Biosafety Level: 1
Availability Status: Discontinued (Please See Comments)
Store at: Room temperature (dry atmosphere)
Contributor: NIAID/NIH
Comments:
NR-18958 is no longer available. DeADMAn DNA Microarray, 12K, Version 2 has a demonstrated shelf-life of one year and is past the expiration date of 10JUN2011. However, if the microarray has been stored under desiccated conditions it is expected to be useful for research purposes for two years or more past the expiration date. The associated Mycobacterium tuberculosis transposon mutant knock-out pools are still available.

The Mycobacterium tuberculosis (M. tuberculosis) DeADMAn (Designer Arrays for Defined Mutant Analysis) DNA microarray provides a simple method used to identify genes that are essential for survival of a stress condition in in vivo model systems of M. tuberculosis infection. A pool of genetically defined transposon mutants (input pool) is used to infect an animal. The infected animal is subjected to a stress condition and the mutants are harvested from the lungs and spleen several weeks postinfection (output pool). Mutants from the input pool and the output pool are identified by amplification of the unique transposon junction sequences of each mutant. The input and output pools are labeled with different fluorescent dyes and hybridized to the DeADMAn microarray. The competitive hybridization of the two pools to the DeADMAn microarray allows for the easy detection of mutants that are present in the input pool and absent from the output pool.

NR-18958 was designed primarily based on M. tuberculosis, strain H37Rv and has 12288 oligonucleotide spots distributed in 48 blocks and includes 392 different oligonucleotides. The arrays are coated with aminosilane. There are 20 transposon mutant knock-out pools available from BEI Resources (NR-15773 to NR-15792) that are companion products to the DeADMAn DNA microarray.
Additional information for the M. tuberculosis DeADMAn DNA microarray, including oligonucleotide sequences, is available at the NCBI Gene Expression Omnibus (GEO) Accession: GPL10551.

NR-18958 is provided as a glass microscope slide printed with 12288 oligonucleotide spots.

The data files for MicroArray Analysis are available for download here.

For additional information see: Lamichhane G., S. Tyagi and W. R. Bishai. "Designer Arrays for Defined Mutant Analysis to Detect Genes Essential for Survival of Mycobacterium tuberculosis in Mouse Lungs." Infect. Immun. 73 (2005): 2533-40. PubMed: 15784600.

Additional information on each pool can be found at:

NR-15773 Mycobacterium tuberculosis Knock-Out Pool 1
NR-15774 Mycobacterium tuberculosis Knock-Out Pool 2
NR-15775 Mycobacterium tuberculosis Knock-Out Pool 3
NR-15776 Mycobacterium tuberculosis Knock-Out Pool 4
NR-15777 Mycobacterium tuberculosis Knock-Out Pool 5
NR-15778 Mycobacterium tuberculosis Knock-Out Pool 6
NR-15779 Mycobacterium tuberculosis Knock-Out Pool 7
NR-15780 Mycobacterium tuberculosis Knock-Out Pool 8
NR-15781 Mycobacterium tuberculosis Knock-Out Pool 9
NR-15782 Mycobacterium tuberculosis Knock-Out Pool 10
NR-15783 Mycobacterium tuberculosis Knock-Out Pool 11
NR-15784 Mycobacterium tuberculosis Knock-Out Pool 12
NR-15785 Mycobacterium tuberculosis Knock-Out Pool 13
NR-15786 Mycobacterium tuberculosis Knock-Out Pool 14
NR-15787 Mycobacterium tuberculosis Knock-Out Pool 15
NR-15788 Mycobacterium tuberculosis Knock-Out Pool 16
NR-15789 Mycobacterium tuberculosis Knock-Out Pool 17
NR-15790 Mycobacterium tuberculosis Knock-Out Pool 18
NR-15791 Mycobacterium tuberculosis Knock-Out Pool 19
NR-15792 Mycobacterium tuberculosis Knock-Out Pool 20
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis DeADMAn DNA Microarray, 12K, Version 2, NR-18958."
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.


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