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NR-36210   Anthrax Edema Factor (EF), Recombinant from Bacillus anthracis
(Toxins)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Anthrax Edema Factor (EF), Recombinant from Bacillus anthracis
Organism: Bacillus anthracis
Biosafety Level: 1
Availability Status: In Stock
Store at: 2°C to 8°C
Contributor: BEI Resources
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval.

Recombinant anthrax edema factor (EF, 89 kDa) was produced using a plasmid licensed from the NIH. The plasmid was introduced into a non-sporulating avirulent strain of Bacillus anthracis lacking both of the wild type plasmids, pX01 and pX02. Recombinant EF was purified using conventional chromatographic techniques. The resulting purified protein lacks all other anthrax virulence factors.

There is one predicted amino acid difference between NR-36210 and NR-141. NR-141 contains an asparagine, rather than a serine, at amino acid position 415 (amino acid position 444 of the precursor protein for NR-141, amino acid position 447 of precursor EF, GenPept P40136, amino acid position 414 of mature EF, GenPept P40136.

There is one expected amino acid difference between NR-36210 and mature EF, GenPept P40136. NR-36210 may have an additional histidine residue at the N-terminus (amino acid position 30 of the precursor protein for NR-36210). This has not yet been confirmed by N-terminal sequencing for NR-36210.

Each vial contains 100 µg of recombinant EF from Bacillus anthracis.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Anthrax Edema Factor (EF), Recombinant from Bacillus anthracis, NR-36210."
Publications Citing
this Reagent:
Arevalo, M. T., et al. "Targeted Silencing of Anthrax Toxin Receptors Protects against Anthrax Toxins." J. Biol. Chem. 289 (2014): 15730-15738. PubMed: 24742682.

Ballinger, M. N., et al. "Transient Increase in Cyclic AMP Localized to Macrophage Phagosomes." PloS One 5 (2010): e13962. PubMed: 21085604.

Gonzales, C. M., et al. "Antibacterial Role for Natural Killer Cells in Host Defense to Bacillus anthracis." Infect. Immun. 80 (2012): 234-242. PubMed: 22006566.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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