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NR-4367   Anthrax Lethal Factor (LF-HMA), Recombinant from Bacillus anthracis
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at no cost to registered researchers.
Description: Anthrax Lethal Factor (LF-HMA), Recombinant from Bacillus anthracis
Organism: Bacillus anthracis
Biosafety Level: 1
Availability Status: Discontinued (Please See Comments)
Store at: 2°C to 8°C
Contributor: BEI Resources
NR-4367, Anthrax Lethal Factor (LF-HMA), Recombinant from Bacillus anthracis, is no longer available.

Recombinant anthrax lethal factor (LF, 90 kDa) was produced using a plasmid licensed from the NIH. The plasmid contains the coding sequences for two additional amino acids, a histidine (H) and a methionine (M), beyond the native N-terminal alanine (A). The lethal factor produced from this plasmid has recently been designated LF-HMA by the manufacturer. The plasmid was introduced into a non-sporulating, avirulent strain of Bacillus anthracis (B. anthracis) lacking both of the wild type plasmids, pX01 and pX02. Recombinant LF-HMA was purified using conventional chromatographic techniques. The resulting purified protein lacks all other anthrax virulence factors.

Each vial contains approximately 0.7 to 0.8 mg (lyophilized) of recombinant LF-HMA from B. anthracis.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Anthrax Lethal Factor (LF-HMA), Recombinant from Bacillus anthracis, NR-4367."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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