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NR-4477  Ricinus communis, Ricin Toxoid, Recombinant with N-Terminal Histidine Tag
(Toxins)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Ricin Toxoid, Recombinant with N-Terminal Histidine Tag
Organism: Ricinus communis
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder (Note: For long term storage, store at -80°C or colder.)
Contributor: AD O'Brien, JF Sinclair
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Ricin toxin is a glycoprotein that can be isolated from the seeds of the castor bean plant Ricinus communis (R. communis). Structurally, ricin toxin consists of two polypeptide chains, A and B, that are linked by a disulfide bond. The A chain of ricin toxin catalytically inactivates the eukaryotic 28S ribosomal RNA subunit resulting in the inhibition of protein synthesis and death of the cell. The ricin toxin B chain is a galactose-specific lectin that mediates the binding and delivery of the toxin to target cells. The sequence of the R. communis gene for the ricin toxin precursor protein has been reported (GenBank: X03179).

NR-4477 is a genetically inactivated ricin toxoid expressed in Escherichia coli and purified by metal affinity chromatography. Catalytic Glu and Arg residues in the toxin active site have been mutagenized to create a non-toxic protein. The protein is expressed recombinantly as a single 62 kDa polypeptide containing both the A and B subunits. It also has an additional amino-terminal histidine-tag sequence.

NR-4477 reacts specifically with monoclonal antibodies to ricin A chain (BEI Resources NR-843) as determined by western blot analysis.

Each vial of NR-4477 contains approximately 500 µg of ricin toxoid suspended in PBS, pH 8.4.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Ricin Toxoid, Recombinant with N-Terminal Histidine Tag, NR-4477."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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