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Product Name:
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Ricin Toxoid, Chemically Inactivated from Ricinus communis
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Ownership statement:
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This reagent is the tangible property of the U.S. Government.
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Manufacturer:
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Alison D. O’Brien, Ph.D., Chairperson, and James F. Sinclair, Ph.D., Laboratory Supervisor, Department of Microbiology and Immunology, Uniformed Services University of the HealthSciences, Bethesda, Maryland, USA
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Material Provided:
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Each vial of NR-4671 contains approximately 1 mg of ricin toxoid suspended in buffer. NR-4671 lots 61632757 and 57680183 contained approximately 1 mg of ricin toxoid suspended in 10 mM Tris buffer (pH ~ 8). The concentration, expressed as mg per mL, is shown on the Certificate of Analysis.
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Packing/Storage:
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NR-4671 was packaged aseptically in cryovials. The product is provided frozen on blue ice and should be stored at -20°C or colder immediately upon arrival. For long term storage, the contributor recommends -80°C or colder.
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Functional Activity:
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NR-4671 reacts with polyclonal antiserum to ricin holotoxin as determined by western blot. In addition, NR-4671, lot 57680183, is reported to react specifically with monoclonal antibodies to ricin A- and B-chains (BEI Resources NR-843 and NR-842).
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Doan, L. G. “Ricin: Mechanism of Toxicity, Clinical Manifestations, and Vaccine Development. A Review.” J. Toxicol. Clin. Toxicol. 42 (2004): 201–208. PubMed: 15214627.
2. Endo, Y. and K. Tsurugi. “RNA N-Glycosidase Activity of Ricin A-chain. Mechanism of Action of the Toxic Lectin Ricin on Eukaryotic Ribosomes.” J. Biol. Chem. 262 (1987): 8128–8130. PubMed: 3036799.
3. Chang, M. S., et al. “Cloning and Expression of Recombinant, Functional Ricin B Chain.” Proc. Natl. Acad. Sci. U.S.A. 84 (1987): 5640–5644. PubMed: 3112772.
4. Olsnes, S., E. Saltvedt, and A. Pihl. “Isolation and Comparison of Galactose-binding Lectins from Abrus precatorius and Ricinus communis.” J. Biol. Chem. 249 (1974): 803–810. PubMed: 4811904.
5. Halling, K. C., et al. “Genomic Cloning and Characterization of a Ricin Gene from Ricinus communis.” Nucleic Acids Res. 13 (1985): 8019–8033. PubMed: 2999712. GenBank: X03179.
6. Fulton, R. J., et al. “Purification of Ricin A1, A2, and B Chains and Characterization of Their Toxicity.” J. Biol. Chem. 261 (1986): 5314–5319. PubMed: 3957927.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Ricin Toxoid, Chemically Inactivated from Ricinus communis, NR-4671."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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