Contact Us   Having technical issues or questions regarding this product?
Please Contact Us.
BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-853  Ricinus communis, Ricin Toxin A Subunit with N-Terminal Histidine Tag, Recombinant from Escherichia coli
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Ricin Toxin A Subunit with N-Terminal Histidine Tag, Recombinant from Escherichia coli
Organism: Ricinus communis
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: AD O'Brien, JF Sinclair
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The sequence of the Ricinus communis gene for the ricin toxin precursor protein has been reported (GenBank: X03179).

NR-853 is a recombinant form of the A subunit of ricin toxin. The amino acid sequence includes an N-terminal histidine tag (MRGSHHHHHHTDPM) and amino acid residues 36 to 302 of the ricin toxin precursor. A QIAGEN pQE-32 vector was used to express the recombinant protein in Escherichia coli. The protein was purified by nickel affinity chromatography. NR-853 has a theoretical molecular weight of approximately 31,636 daltons.

NR-853 reacts specifically with polyclonal antibody to ricin holotoxin (BEI Resources NR-862), polyclonal antibody to ricin A chain (BEI Resources NR-863), and monoclonal antibody to ricin A chain (BEI Resources NR-843) as determined by Western blot analysis. NR-853 is not active in an in vitro cytotoxicity assay using Vero cells.

Each vial of NR-853 contains approximately 1 mg of recombinant ricin toxin A chain suspended in 10 mM sodium acetate buffer (pH 5.6).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Ricin Toxin A Subunit with N-Terminal Histidine Tag, Recombinant from Escherichia coli, NR-853."
Publications Citing
this Reagent:
Li, X.-P., et al. "Pentameric Organization of the Ribosomal Stalk Accelerates Recruitment of Ricin A Chain to the Ribosome for Depurination." J. Biol. Chem. 285 (2010): 41463-41471. PubMed: 20974854.
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Click to read more
Return to Top

Notices and Disclaimers

BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.

Back to My Search