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NR-853  Ricinus communis, Ricin Toxin A Subunit with N-Terminal Histidine Tag, Recombinant from Escherichia coli
(Toxins)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Ricin Toxin A Subunit with N-Terminal Histidine Tag, Recombinant from Escherichia coli
Organism: Ricinus communis
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: AD O'Brien, JF Sinclair
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The sequence of the Ricinus communis gene for the ricin toxin precursor protein has been reported (GenBank: X03179).

NR-853 is a recombinant form of the A subunit of ricin toxin. The amino acid sequence includes an N-terminal histidine tag (MRGSHHHHHHTDPM) and amino acid residues 36 to 302 of the ricin toxin precursor. A QIAGEN pQE-32 vector was used to express the recombinant protein in Escherichia coli. The protein was purified by nickel affinity chromatography. NR-853 has a theoretical molecular weight of approximately 31,636 daltons.

NR-853 reacts specifically with polyclonal antibody to ricin holotoxin (BEI Resources NR-862), polyclonal antibody to ricin A chain (BEI Resources NR-863), and monoclonal antibody to ricin A chain (BEI Resources NR-843) as determined by Western blot analysis. NR-853 is not active in an in vitro cytotoxicity assay using Vero cells.

Each vial of NR-853 contains approximately 1 mg of recombinant ricin toxin A chain suspended in 10 mM sodium acetate buffer (pH 5.6).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Ricin Toxin A Subunit with N-Terminal Histidine Tag, Recombinant from Escherichia coli, NR-853."
Publications Citing
this Reagent:
Li, X.-P., et al. "Pentameric Organization of the Ribosomal Stalk Accelerates Recruitment of Ricin A Chain to the Ribosome for Depurination." J. Biol. Chem. 285 (2010): 41463-41471. PubMed: 20974854.
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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