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The sequence of the gene for the epsilon toxin precursor protein has been reported (GenBank: M95206). The structure of epsilon prototoxin has been solved (PDB: 1UYJ).NR-855 is a recombinant form of epsilon toxin from Clostridium perfringens. The amino acid sequence includes an N-terminal histidine tag (MRGSHHHHHHG), amino acid residues 40 to 324 of the epsilon toxin precursor protein, and five plasmid-encoded amino acids at the C-terminus. A QIAGEN pQE30 vector was used to express the recombinant protein in Escherichia coli. The protein was purified by nickel affinity chromatography. NR-855 has a theoretical molecular weight of approximately 33,271 daltons.NR-855 reacts with polyclonal IgG antibody produced by immunization of rabbits with peptides that correspond to distinct internal regions of the full-length epsilon toxin (BEI Resources NR-865).Each vial of NR-855 contains approximately 1 mg of recombinant epsilon toxin suspended in 10 mM sodium phosphate buffer (pH 7.4).For information about toxin amounts excluded from CDC Select Agent regulations, click here.
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
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Do NOT reference BEI Resources or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
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as they appear on your BEI Resources registration.
Information about permits is provided as a courtesy to BEI Resources customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by BEI Resources, please select this link: Compliance Requirements.
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