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NR-855   Epsilon Toxin from Clostridium perfringens with N-Terminal Histidine Tag, Recombinant from Escherichia coli
(Toxins)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Epsilon Toxin from Clostridium perfringens with N-Terminal Histidine Tag, Recombinant from Escherichia coli
Organism: Clostridium perfringens
Biosafety Level: 2
Availability Status: In Stock
Store at: -20°C or colder
Contributor: AD O'Brien, JF Sinclair
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.


The sequence of the gene for the epsilon toxin precursor protein has been reported (GenBank: M95206). The structure of epsilon prototoxin has been solved (PDB: 1UYJ).

NR-855 is a recombinant form of epsilon toxin from Clostridium perfringens. The amino acid sequence includes an N-terminal histidine tag (MRGSHHHHHHG), amino acid residues 40 to 324 of the epsilon toxin precursor protein, and five plasmid-encoded amino acids at the C-terminus. A QIAGEN pQE30 vector was used to express the recombinant protein in Escherichia coli. The protein was purified by nickel affinity chromatography. NR-855 has a theoretical molecular weight of approximately 33,271 daltons.

NR-855 reacts with polyclonal IgG antibody produced by immunization of rabbits with peptides that correspond to distinct internal regions of the full-length epsilon toxin (BEI Resources NR-865).

Each vial of NR-855 contains approximately 1 mg of recombinant epsilon toxin suspended in 10 mM sodium phosphate buffer (pH 7.4).

For information about toxin amounts excluded from CDC Select Agent regulations, click here.

Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Epsilon Toxin from Clostridium perfringens with N-Terminal Histidine Tag, Recombinant from Escherichia coli, NR-855."
Additional Information: NR-855 Safety Data Sheet
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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