Back to My Search

Login is required to view Product Sheets and Certificates of Analysis.

Contact Us   Having technical issues or questions regarding this product?
Please Contact Us.
BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-855   Epsilon Toxin from Clostridium perfringens with N-Terminal Histidine Tag, Recombinant from Escherichia coli
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Epsilon Toxin from Clostridium perfringens with N-Terminal Histidine Tag, Recombinant from Escherichia coli
Organism: Clostridium perfringens
Biosafety Level: 2
Availability Status: In Stock
Store at: -20°C or colder
Contributor: AD O'Brien, JF Sinclair
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The sequence of the gene for the epsilon toxin precursor protein has been reported (GenBank: M95206). The structure of epsilon prototoxin has been solved (PDB: 1UYJ).

NR-855 is a recombinant form of epsilon toxin from Clostridium perfringens. The amino acid sequence includes an N-terminal histidine tag (MRGSHHHHHHG), amino acid residues 40 to 324 of the epsilon toxin precursor protein, and five plasmid-encoded amino acids at the C-terminus. A QIAGEN pQE30 vector was used to express the recombinant protein in Escherichia coli. The protein was purified by nickel affinity chromatography. NR-855 has a theoretical molecular weight of approximately 33,271 daltons.

NR-855 reacts with polyclonal IgG antibody produced by immunization of rabbits with peptides that correspond to distinct internal regions of the full-length epsilon toxin (BEI Resources NR-865).

Each vial of NR-855 contains approximately 1 mg of recombinant epsilon toxin suspended in 10 mM sodium phosphate buffer (pH 7.4).

For information about toxin amounts excluded from CDC Select Agent regulations, click here.

Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Epsilon Toxin from Clostridium perfringens with N-Terminal Histidine Tag, Recombinant from Escherichia coli, NR-855."
Additional Information: NR-855 Safety Data Sheet
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Return to Top

Notices and Disclaimers

BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.

Back to My Search