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The sequence of the gene for the epsilon toxin precursor protein has been reported (GenBank: M95206). The structure of epsilon prototoxin has been solved (PDB: 1UYJ).NR-855 is a recombinant form of epsilon toxin from Clostridium perfringens. The amino acid sequence includes an N-terminal histidine tag (MRGSHHHHHHG), amino acid residues 40 to 324 of the epsilon toxin precursor protein, and five plasmid-encoded amino acids at the C-terminus. A QIAGEN pQE30 vector was used to express the recombinant protein in Escherichia coli. The protein was purified by nickel affinity chromatography. NR-855 has a theoretical molecular weight of approximately 33,271 daltons.NR-855 reacts with polyclonal IgG antibody produced by immunization of rabbits with peptides that correspond to distinct internal regions of the full-length epsilon toxin (BEI Resources NR-865).Each vial of NR-855 contains approximately 1 mg of recombinant epsilon toxin suspended in 10 mM sodium phosphate buffer (pH 7.4).For information about toxin amounts excluded from CDC Select Agent regulations, click here.
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biological materials provided by BEI Resources, please select this link: Compliance Requirements.
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