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NR-859   Shiga Toxin Type 1 Subunit A, Recombinant from Escherichia coli
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Shiga Toxin Type 1 Subunit A, Recombinant from Escherichia coli
Organism: Escherichia coli
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: AD O'Brien, JF Sinclair
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-859 is a recombinant protein of the Shiga toxin type 1 subunit A (rStx1A). The protein sequence of the native Shiga toxin A subunit was modified to include an internal penta-histidine-tag used for the purpose of purification. The tagged protein was produced using a Qiagen QE30 series his-tag vector series, expressed in E. coli, and purified by nickel affinity chromatography. The resulting protein, NR-859, has a molecular weight of approximately 34,900 daltons. The predicted protein sequence of NR-859 is shown in Table 1 on the product sheet.

Each vial contains 0.1 mg of NR-859 suspended in phosphate buffered saline.

NR-859 reacts with rabbit polyclonal antiserum specific for Stx type 1 subunit A. Carbonic anhydrase negative control is not recognized.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Shiga Toxin Type 1 Subunit A, Recombinant from Escherichia coli, NR-859."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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