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NR-860   Shiga Toxin Type 1 Subunit B, Recombinant from Escherichia coli
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Shiga Toxin Type 1 Subunit B, Recombinant from Escherichia coli
Organism: Escherichia coli
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: AD O'Brien, JF Sinclair
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-860 is a recombinant form of the B subunit of Shiga toxin type 1 (Stx1). The amino acid sequence includes a C-terminal hexa-histidine tag and amino acid residues 21 to 89 of the Stx1 subunit B protein (GenPept: AAA98348). The recombinant protein was expressed in Escherichia coli and purified by nickel affinity chromatography. NR-860 has a theoretical molecular weight of approximately 8,513 daltons.

Each vial of NR-860 contains approximately 1 mg of recombinant Stx1 subunit B suspended in phosphate buffered saline (pH 7.4). NR-860 lot 57680170 contains approximately 0.1 mg per vial suspended in phosphate buffered saline (pH 7.4).

NR-860 reacts with mouse monoclonal antibody to Shiga toxin type 1 subunit B (BEI Resources NR-844).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Shiga Toxin Type 1 Subunit B, Recombinant from Escherichia coli, NR-860."
Publications Citing
this Reagent:
Huang, J., et al. "The B Subunits of Shiga-Like Toxins Induce Regulated VWF Secretion in a Phospholipase D1-Dependent Manner." Blood 120 (2012): 1143-1149. PubMed: 22718838.

Huang, J., et al. "Shiga Toxin B Subunits Induce VWF Secretion by Human Endothelial Cells and Thrombotic Microangiopathy in ADAMTS13-Deficient Mice." Blood 116 (2010): 3653-3659. PubMed: 20644116.

Liu, F., et al. "Shiga Toxin (Stx) 1B and Stx2B Induce von Willebrand Factor Secretion from Human Umbilical Vein Endothelial Cells through Different Signaling Pathways." Blood 118 (2011): 3392-3398. PubMed: 21816831.

Rumah, K. R., et al. "Isolation of Clostridium perfringens Type B in an Individual at First Clinical Presentation of Multiple Sclerosis Provides Clues for Environmental Triggers of the Disease." PloS One 8 (2013): e76359. PubMed: 24146858.
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.

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