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Plasmodium falciparum piggyBac Insertion Mutants Now Available
From the laboratory of John Adams, we now have over 100 piggyBac insertion mutants available, complete with mapped insertion sites. The list of currently available clones is HERE. Please place your request for individual mutants by specifying the MR4 number (column 1 on the spreadsheet) and Clone ID number (column 4) to malaria@atcc.org. Clones will be listed as separate reagents by MR4 number in the MR4 and BEI Resources catalogs in the near future.
Balu B, et al. "High-efficiency transformation of Plasmodium falciparum by the lepidopteran transposable element piggyBac." Proc Natl Acad Sci USA. 102: 16391-16396, 2005. PubMed: 16260745.
Balu B, et al. "piggyBac is an effective tool for functional analysis of the Plasmodium falciparum genome." BMC Microbiol. 9: 83, 2009. PubMed: 19422698.
Balu B, et al. "A genetic screen for attenuated growth identifies genes crucial for intraerythrocytic development of Plasmodium falciparum." PLoS One. 5: e13282, 2010. PubMed: 20949012.