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Product Name:
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F0402
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Spirochaetaceae, Treponema
Species: Treponema denticola
Strain: F0402 (formerly known as B1)
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Additional Information:
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T. denticola is a Gram-negative, obligately anaerobic, nonsporulating, motile spirochete usually found in the microflora of a human mouth, predominately in the subgingival plaque of patients with periodontitis.2 This invasive bacterium has been identified as an important cause of periodontal disease and suspected to be involved in extra-oral infections.3,4,5,6
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Modified New Oral Spirochete broth supplemented with 10% DMSO.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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HM-259 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Modified New Oral Spirochete broth or equivalent
Note: Growth on agar is not recommended.
Incubation:
Temperature: 37°C
Atmosphere: Anaerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate additional broth tubes.
4. Incubate cultures at 37°C for 5 to 7 days.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use.
Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org.
While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate.
This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products.
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References:
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1. Izard, J., personal communication
2. Chan, E. C., et al. “Treponema denticola (ex Brumpt 1925) sp. nov., nom. rev., and Identification of New Spirochete Isolates from Periodontal Pockets.” Int. J. Syst. Bacteriol. 43 (1993): 196-203. PubMed: 8494734.
3. Dashper, S. G., et al. “Virulence Factors of the Oral Spirochete Treponema denticola.” J. Dent. Res. 90 (2011): 691-703. PubMed: 20940357.
4. Frederick, J. R., et al. “Molecular Signaling Mechanisms of the Periopathogen, Treponema denticola.” J. Dent. Res. 90 (2011): 1155-1163. PubMed: 21447698.
5. Gaibani, P., et al. “Killing of Treponema denticola by Mouse Peritoneal Macrophages.” J. Dent. Res. 89 (2010): 521-526. PubMed: 20200417.
6. Sela, M. N. “Role of Treponema denticola in Periodontal Diseases.” Crit. Rev. Oral. Biol. Med. 12 (2001): 399-413. PubMed: 12002822.
7. Izard, J., et al. “Native Cellular Architecture of Treponema denticola Revealed by Cryo-Electron Tomography.” J. Struct. Biol. 163 (2008): 10-17. PubMed: 18468917.
8. Seshadri, R., et al. “Comparison of the Genome of the Oral Pathogen Treponema denticola with Other Spirochete Genomes.” Proc. Natl. Acad. Sci. USA. 101 (2004): 5646-5651. PubMed: 15064399.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH as part of the Human Microbiome Project: Treponema denticola, Strain F0402, HM-259."
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Biosafety Level:
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1
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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