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Genomic DNA was isolated from a preparation of Burkholderia pseudomallei (B. pseudomallei), strain 1026b.Burkholderia pseudomallei (B. pseudomallei), strain 1026b was isolated in 1993 from a blood culture of a female rice farmer with diabetes mellitus at Sappasithiprasong hospital in Ubon, Ratchathani, Thailand. The patient was admitted with septicemic melioidosis with soft tissue, skin, joint and splenic involvement, and survived to discharge. B. pseudomallei was isolated from pus and blood samples, strains 1026a and 1026b, respectively, that were obtained 14 days after the onset of symptoms.The genome sequences of chromosomes 1 and 2 of B. pseudomallei, strain 1026b are available (GenBank: NC_017831.1 and NC_017832.1). Genome sequences obtained from strains 1026a and 1026b are reported to be identical. B. pseudomallei, strain 1026b contains a phage, phi1026b, which has been sequenced (GenBank: AY453853).NR-9321 has been qualified for PCR applications by amplification of approximately 1500 base pairs of the 16S ribosomal RNA gene.Each vial contains 0.7 µg to 1.5 µg of bacterial genomic DNA in 10 mM Tris-HCl, pH 8 – 8.5. Each vial of lot 57960661 contains 4 µg to 6 µg of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 7.4). The vial should be centrifuged prior to opening.
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