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Transgenic

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Transgenic
Strain Organism MRA No. Notes Thumbnail
P-BAC-0     
A. gambiae
335
eGFP expression particularly in the anterior stomach and distal lobes of salivary glands; Contains pK[BIGalpha] insertion in 28D. See Genbank accession numbers AF402294 and AF402295
IV
A. stephensi
314
L4s of this stock showed GFP expression primarily in gastric cecae and the posterior stomach
SRtTA
A. stephensi
728
SRtTA is homozygous for Actin 5C-eGFP marker with an autosomal insertion of a reverse tTA which is controlled by a serpin 10 promoter fragment. Progeny crosses to a LacZ reponder line reveal staining in the midgut, hemocytes and pericardial cells of larvae and adults.
StTA/ tetOPLacZ
A. stephensi
727
This stock has an X-linked tetracycline transactivator gene driver (tTA1) which is controlled by a seprin 10 promoter fragment. Regulation of this system is done via doxycycline. This strain is double marked with an Actin 5c-eGFP and an Actin 5C-dsRed1 marker. Lines carrying individual insertions should be isolated following outcrossing to wild type A. stephensi.
B1
A. stephensi
855
RNA silencing of eGFP transcripts in transgenic A. stephensi mosquitoes.  pIR-EGFP insertion with dsRed marker under Actin5C promoter.  Parental crosses result in 43% reduction in GFP expression.  Backcrosses of progeny to B1 males results in 73% reduction in expression.
19.1
A. stephensi
854
RNA silencing of eGFP transcripts in transgenic A. stephensi mosquitoes. pIR-EGFP insertion with dsRed marker under Actin5C promoter.  Parental crosses result in a 37% reduction in GFP expression.  Backcrosses of progeny to B1 males results in a 65% reduction in expression.

 

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