Isolates of Trypanosoma brucei Used in In Vivo Research

 

The African trypanosomes infect a broad range of mammals across sub-Saharan Africa and result in significant losses to domestic livestock. Subspecies of Trypanosoma brucei also cause African trypanosomiasis in humans resulting in approximately 20,000 cases per year.1 The development of vaccines against this disease is extremely difficult due to high antigenic variation of trypanosome surface glycoproteins, and a rise in resistance to trypanocidal drugs has been documented.2,3 Mouse models of infection and drug susceptibility are critical to understanding the mechanisms of disease and the development of new therapeutic drugs.

BEI Resources houses several isolates of T. brucei to support in vivo research on African trypanosomiasis. T. brucei subsp. gambiense, strain 348BT (NR-53513) is a recent acquisition that was isolated from a patient in the Democratic Republic of Congo before receiving treatment with melarsoprol that displays reduced sensitivity to the drug in laboratory mice.4,5

Other African trypanosome acquisitions in BEI Resources catalog include the reference T. brucei subsp. brucei, strain TREU 667 (NR-46440), as well as a variety of clinical isolates used in drug susceptibility studies in vivo. For more information on the availability of these cultures, please visit the BEI Resources website or contact Customer Care at contact@beiresources.org.

 

BEI Resources 

Product Description

NR-36197

Trypanosoma brucei subsp. brucei, Strain STIB 247

NR-36198

Trypanosoma brucei subsp. gambiense, Strain STIB 386

NR-46431

Trypanosoma brucei subsp. rhodesiense, Strain KETRI 243

NR-46432

Trypanosoma brucei subsp. rhodesiense, Strain KETRI 269

NR-46433

Trypanosoma brucei subsp. rhodesiense, Strain KETRI 2285

NR-46434

Trypanosoma brucei subsp. rhodesiense, Strain KETRI 2482

NR-46435

Trypanosoma brucei subsp. rhodesiense, Strain KETRI 2538

NR-46436

Trypanosoma brucei subsp. rhodesiense, Strain KETRI 2537

NR-46437

Trypanosoma brucei subsp. rhodesiense, Strain KETRI 2562

NR-46438

Trypanosoma brucei subsp. rhodesiense, Strain KETRI 2772

NR-46439

Trypanosoma brucei subsp. brucei, Strain Lab 110 EATRO

NR-46440

Trypanosoma brucei subsp. brucei, TREU 667

NR-53513

Trypanosoma brucei subsp. gambiense, Strain 348BT

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

References:

  1. Brun, R., et al. “Human African Trypanosomiasis.” Lancet 375 (2010): 148-159. PubMed: 19833383.
  2. B├╝scher, P., et al. “Human African Trypanosomiasis.” Lancet 390 (2017): 2397-2409. PubMed: 28673422.
  3. “Trypanosomiasis, Human African (Sleeping Sickness).” World Health Organization, https://www.who.int/news-room/fact-sheets/detail/trypanosomiasis-human-african-(sleeping-sickness). Accessed 29 July 2021.
  4. Pyana, P. P., et al. “Isolation of Trypanosoma brucei gambiense from Cured and Relapsed Sleeping Sickness Patients and Adaptation to Laboratory Mice.” PLoS Negl. Trop. Dis. 5 (2011): e1025. PubMed: 21526217.
  5. Pyana, P. P., et al. “Melarsoprol Sensitivity Profile of Trypanosoma brucei gambiense Isolates from Cured and Relapsed Sleeping Sickness Patients from the Democratic Republic of Congo.” PLoS Negl. Trop. Dis. 8 (2014): e3212. PubMed: 25275572.

Image:  Photomicrograph of Trypanosoma brucei parasites in a Giemsa-stained blood sample (CDC/Blaine Mathison)

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