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NR-2640   Genomic DNA from Vaccinia Virus, Western Reserve (Mouse adapted)
(Nucleic Acids)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Genomic DNA from Vaccinia Virus, Western Reserve (Mouse adapted)
Organism: Vaccinia virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: ATCC®
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Genomic DNA was isolated from a preparation of cell lysate and supernatant from African green monkey cells (ATCC ® CRL-2378.1™) infected with vaccinia virus, Western Reserve (WR; mouse adapted; BEI Resources NR-56).

The WR (mouse adapted) strain was derived from the original New York City Board of Health (NYCBH) strain by intracerebral passages in mice. It has been utilized in constructing vectors for gene expression and in producing viral proteins and DNA. The complete genomic sequence of vaccinia virus, WR has been determined (GenBank: AY243312).

NR-2640 has been qualified for PCR applications by amplification of a sequence of at least 1000 bp. NR-2640 is not intended for use as a standard for quantitative PCR.

Each vial contains 100 µL of viral genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0). The viral genomic DNA is in a background of cellular nucleic acid and carrier RNA. The vial should be centrifuged prior to opening.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Vaccinia Virus, Western Reserve (Mouse adapted), NR-2640."
Publications Citing
this Reagent:
Baker, J. L., and B. M. Ward. "Development and Comparison of a Quantitative TaqMan-MGB Real-Time PCR Assay to Three Other Methods of Quantifying Vaccinia Virions." J. Virol. Methods 196 (2014): 126-132. PubMed: 24211297.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
NR-56
Vaccinia virus Western Reserve (Mouse adapted)
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