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NR-2641   Genomic DNA from Cowpox Virus, Brighton Red
(Nucleic Acids)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Genomic DNA from Cowpox Virus, Brighton Red
Organism: Cowpox virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: ATCC®
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Genomic DNA was extracted from a preparation of cell lysate and supernatant from African green monkey cells (ATCC ® CRL-26™) infected with cowpox virus, Brighton Red (BEI Resources NR-88).

The Brighton strain was derived from a lesion on the finger of a cowman on a farm near Brighton, England in 1937. It has been passaged multiple times in guinea pigs and rabbits. The complete genomic sequence of cowpox virus, Brighton Red has been determined (GenBank: AF482758).

NR-2641 has been qualified for PCR applications by amplification of an approximately 1000 nucleotide sequence.

Each vial contains 100 µL of viral genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0). The viral genomic DNA is in a background of cellular nucleic acid and carrier RNA. The vial should be centrifuged prior to opening.

Each vial of NR-2641 lot 5075337, which is no longer available, contained a target amount of 1 × 108 copies of viral genomic DNA in TE buffer (pH 7.5) containing sodium azide.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Cowpox Virus, Brighton Red, NR-2641."
Publications Citing
this Reagent:
Baker, J. L., and B. M. Ward. "Development and Comparison of a Quantitative TaqMan-MGB Real-Time PCR Assay to Three Other Methods of Quantifying Vaccinia Virions." J. Virol. Methods 196 (2014): 126-132. PubMed: 24211297.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
NR-88
Cowpox virus Brighton Red
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