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NR-2652   Genomic DNA from Escherichia coli, Strain NCDC 909-51
(Nucleic Acids)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Genomic DNA from Escherichia coli, Strain NCDC 909-51
Organism: Escherichia coli
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C
Contributor: ATCC®
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Genomic DNA was isolated from a preparation of Escherichia coli (E. coli), strain NCDC 909-51, serotype O28a,28c:K73(B18):NM. The bacterial preparation was produced by propagation of BEI Resources NR-101.

The enteroinvasive E. coli (EIEC) strain NCDC 909-51 was isolated from the feces of a patient in Katwijk, The Netherlands around 1940. EIEC strains invade and multiply within intestinal epithelial cells, resulting in a dysentery-like enteritis in humans, similar to that caused by Shigella species. EIEC pathogenesis requires the expression of genes present both on the chromosome and on a large invasion plasmid, pINV (220,000 bp). The plasmid shares a significant degree of DNA homology with the virulence plasmid described in Shigella species, and is structurally and functionally equivalent.

NR-2652 has been qualified for PCR applications by amplification of approximately 1500 bp of the 16S ribosomal RNA gene.

Each vial contains 4-6 micrograms of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~7.4). The vial should be centrifuged prior to opening.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Escherichia coli, Strain NCDC 909-51, NR-2652."
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
NR-101
Escherichia coli NCDC 909-51 (Serotype O28a,O28c:K73(B18):NM)
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