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NR-13298   Plasmid pMRLB.47 Containing Gene Rv1886c (Protein Ag85B) from Mycobacterium tuberculosis
(Plasmid/Vectors)
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at no cost to registered researchers.
Description: Plasmid pMRLB.47 Containing Gene Rv1886c (Protein Ag85B) from Mycobacterium tuberculosis
Organism: Mycobacterium tuberculosis
Biosafety Level: 1
Availability Status: Discontinued (Please See Comments)
Store at: -20°C or colder
Contributor: NIH - TB Vaccine Testing and Research Materials Contract
Comments:
NR-13298, Plasmid pMRLB.47 Containing Gene Rv1886c (Protein Ag85B) from Mycobacterium tuberculosis, is no longer available.

NR-13298 is a recombinant expression vector containing Mycobacterium tuberculosis gene Rv1886c, which is a precursor of the 85-B antigen, also referred to as a fibronectin-binding protein (FpbB), that is involved in cell wall mycoloylation. Gene Rv1886c was amplified by PCR and cloned into pET23b for expression in Escherichia coli. The gene was cloned without a signal sequence. The expressed protein is histidine-tagged and has an observed molecular weight of 32 kDa. The expected purified protein yield from a one liter culture is approximately 1 mg.

Note: Plasmid pMRLB.47 contains the gene required for ampicillin (Ap) resistance. The recommended concentration of Ap in culture is 130 µg/mL.

Each vial contains approximately 1 µg of plasmid DNA in 10 mM Tris-HCl, pH 8.5. Lot 04.EC.12.01 contains approximately 1 µg of plasmid DNA in 10 mM Tris-HCl, pH 7.5.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pMRLB.47 Containing Gene Rv1886c (Protein Ag85B) from Mycobacterium tuberculosis, NR-13298."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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