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NR-46154   Escherichia coli - Staphylococcus aureus Shuttle Vector pCN54, Recombinant in Escherichia coli
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Escherichia coli - Staphylococcus aureus Shuttle Vector pCN54, Recombinant in Escherichia coli
Organism: Escherichia coli
Biosafety Level: 1
Availability Status: In Stock
Store at: -60°C or colder
Contributor: RP Novick
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-46154 is a culture of Escherichia coli (E. coli) DH5a (RN9619, NRS619) containing the E. coli-staphylococcal shuttle vector pCN54. Vector pCN54 contains the E. coli ColE1 replication origin and the Staphylococcus aureus (S. aureus) pT181 cop-wt-repC replicon, the cadmium-inducible promoter Pcad-cadC, the GFP reporter gene gfpmut2 and the blaZ transcriptional terminator. Vector pCN54 was deposited as resistant to ampicillin and erythromycin in E. coli and resistant to erythromycin in S. aureus.

The complete sequence and vector map of pCN54 have been determined and are available on the Certificate of Analysis for lot 63381374. The BEI Resources vector sequence was deposited into GenBank as NR-46154 (GenBank: KR781470).

Each vial of NR-46154 contains approximately 0.5 mL of bacterial culture in Luria-Bertani (LB) broth containing 100 µg/mL ampicillin supplemented with 10% glycerol.
Citations: Acknowledgment for publications should read "The following reagent was provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) for distribution by BEI Resources, NIAID, NIH: Escherichia coli - Staphylococcus aureus Shuttle Vector pCN54, Recombinant in Escherichia coli, NR-46154."
Additional Information: NARSA NRS619
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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