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NR-50348   Escherichia coli - Staphylococcus aureus Shuttle Vector pKK22, Recombinant in Escherichia coli
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Description: Escherichia coli - Staphylococcus aureus Shuttle Vector pKK22, Recombinant in Escherichia coli
Organism: Escherichia coli
Biosafety Level: 1
Availability Status: In Stock
Store at: -60°C or colder
Contributor: JL Bose, EV Stabb
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-50348 is a preserved culture of Escherichia coli (E. coliDH5αλpir containing the E. coli-staphylococcal shuttle vector pKK22. Vector pKK22 contains the E. coli R6Kγ origin of replication and is for use in E. coli and Staphylococcus aureus (S. aureus) USA300 strains that contain LAC-p01, rendering them isogenic. Vector pKK22 contains a single trimethoprim resistance cassette that is functional in both E. coli and S. aureus. The complete pKK22 nucleotide sequence is available (GenBank: KX085042) and the vector map of pKK22 is available on the Product Information Sheet.

pKK22 was deposited in conjunction with pKK30 and E. coli strains DH5αλpir and GM2163λpir (available as a kit, BEI Resources NR-50352). pKK22 and pKK30 were created to maintain stability in E. coli and Staphylococcus species without antibiotic selection during in vitro and in vivo experiments. The E. coli R6Kγ origin of replication of both vectors requires pir+ for replication which is provided in either DH5αλpir or GM2163λpir E. coli strains.

Catalog Number
Vector or Host
NR-50348 pKK22 For use in E. coli DH5αλpir or GM2163λpir or S. sureus USA300 strains containing LAC-p01
NR-50349 pKK30 pKK30 is a variant of pKK22, for use in E. coli DH5αλpir or GM2163λpir or Staphylococcus species not containing LAC-p01
NR-50350 E. coli DH5αλpir Host strain containing the pir genes for performing genetic manipulations prior to transfer into Staphylococcus (F-ϕ80dlacZ ΔM15 ΔlacZYA-argF U169 deoR supE44 hsdR17 recA1 endA1 gyrA96 thi-1 relA1)
NR-50351 E. coli GM2163λpir Host strain containing the pir genes for performing genetic manipulations. This strain is also a dam and dcm methylase mutant for transfer of plasmids into Staphylococcus isolates that do not accept E. coli DNA easily (F-ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 rpsL136 dam13::Tn9 xylA5 mtl-1 thi-1 mcrB1 hsdR2 λpir)

This strain was developed by Krute, et al., at the Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, PubMed:  27637878.

Each vial of NR-50348 contains approximately 0.5 mL of E. coliDH5αλpir, in Tryptic Soy broth containing 10 µg/mL trimethoprim supplemented with 10% glycerol.

Citations: Acknowledgment for publications should read “The following reagent was contributed by Dr. J. L Bose for distribution by BEI Resources, NIAID, NIH: Escherichia coliStaphylococcus aureus Shuttle Vector pKK22, Recombinant in Escherichia coli, NR-50348.”
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Escherichia coli - Staphylococcus aureus Shuttle Vector pKK30, Recombinant in Escherichia coli
Escherichia coli DH5αλpir
Escherichia coli GM2163λpir
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