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NR-52974 Vector pLVX-EF1α-IRES-Puro Containing the SARS-Related Coronavirus 2, USA-WA1/2020 Open Reading Frame 9b Gene
(Plasmid/Vectors)

Price: All BEI Resources products are provided
at no cost to registered researchers.

Description:

Vector pLVX-EF1α-IRES-Puro Containing the SARS-Related Coronavirus 2, USA-WA1/2020 Open Reading Frame 9b Gene

Organism:

SARS-Related Coronavirus 2

Biosafety Level:

Availability Status:

In Stock

Store at:

-20°C or colder

Contributor:

N Krogan

Comments:

Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Note: The vial label indicates this product contains a TST tag. This nomenclature refers to a 2X Strep tag. This product does not express the Twin-Strep-tag^® that is commonly referred to as a TST tag.

The open reading frame 9b (orf9b) gene from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), USA-WA1/2020 (GenBank: MN985325) was codon optimized and modified by the addition of a C-terminal 2X Strep tag and cloned into the pLVX-EF1α-IRES-Puro lentiviral expression plasmid. The vector contains an internal ribosomal entry site (IRES) that allows a gene-of-interest and a puromycin resistance gene to be simultaneously co-expressed from a single mRNA transcript. Expression of the transcript is driven by the human elongation factor 1 alpha (EF1α) promoter. The beta-lactamase gene, bla, provides transformant selection through ampicillin resistance in Escherichia coli (E. coli) and the puromycin resistance gene, pac, provides transformant selection through puromycin resistance in eukaryotic cells. The resulting size of the plasmid is approximately 9200 base pairs. NR-52974 can be used for transient expression and lentivirus generation. The complete plasmid sequence and map are provided on the BEI Resources webpage. The plasmid was produced in E. coli and extracted.

ORF9b is an alternative open reading frame within the nucleocapsid (N) gene of SARS-CoV-2. It has been shown in SARS-like coronaviruses to target mitochondria and significantly inhibit interferon I production.

Each vial contains plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). The vial should be centrifuged prior to opening. Note: The contents of the vial should be used to replicate the plasmid in E. coli prior to mammalian expression studies.

Citations:

Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Vector pLVX-EF1α-IRES-Puro Containing the SARS-Related Coronavirus 2, USA-WA1/2020 Open Reading Frame 9b Gene, NR-52974.”

Restrictions On Use:

This material is distributed for internal research, noncommercial purposes only. This material, its product or its derivatives may not be distributed to third parties. Except as performed under a U.S. Government contract, individuals contemplating commercial use of the material, its products or its derivatives must contact the contributor to negotiate a license. U.S. Government contractors may need a license before first commercial sale.

Product Name:

Vector pLVX-EF1α-IRES-Puro Containing the SARS-Related Coronavirus 2, USA-WA1/2020 Open Reading Frame 9b Gene

Disclaimers:

You are authorized to use this product for research use only. It is not intended for human use.

Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC^® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC^® nor the U.S. Government warrants that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC^® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC^®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products.

Citation:

Biosafety Level:

1 Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.

Product Information Sheet

Certificate of Analysis by Lot

70037927

Other

Plasmid Sequence and Map

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Notices and Disclaimers

BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.

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Product Highlights for NR-52974

Product Sheet Information for NR-52974 Vector pLVX-EF1α-IRES-Puro Containing the SARS-Related Coronavirus 2, USA-WA1/2020 Open Reading Frame 9b Gene, SARS-Related Coronavirus 2, For research use only. Not for use in humans.

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